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Traceability of Plant Diet Contents in Raw Cow Milk Samples
Institute of Agricultural Biology and Biotechnology (IBBA), Italian National Research Council (CNR), Milan 20133, Italy
* Author to whom correspondence should be addressed.
Received: 9 October 2009; Accepted: 1 December 2009 / Published: 4 December 2009
Abstract: The use of molecular marker in the dairy sector is gaining large acceptance as a reliable diagnostic approach for food authenticity and traceability. Using a PCR approach, the rbcL marker, a chloroplast-based gene, was selected to amplify plant DNA fragments in raw cow milk samples collected from stock farms or bought on the Italian market. rbcL-specific DNA fragments could be found in total milk, as well as in the skimmed and the cream fractions. When the PCR amplified fragments were sent to sequence, the nucleotide composition of the chromatogram reflected the multiple contents of the polyphytic diet.
Keywords: DNA detection; traceability; feed
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Ponzoni, E.; Mastromauro, F.; Gianì, S.; Breviario, D. Traceability of Plant Diet Contents in Raw Cow Milk Samples. Nutrients 2009, 1, 251-262.
Ponzoni E, Mastromauro F, Gianì S, Breviario D. Traceability of Plant Diet Contents in Raw Cow Milk Samples. Nutrients. 2009; 1(2):251-262.
Ponzoni, Elena; Mastromauro, Francesco; Gianì, Silvia; Breviario, Diego. 2009. "Traceability of Plant Diet Contents in Raw Cow Milk Samples." Nutrients 1, no. 2: 251-262.