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• Farnfield, MM.
• Carey, KA.
• Gran, P
• Trenerry, MK.
• Cameron-Smith, D
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• Farnfield, MM.
• Carey, KA.
• Gran, P
• Trenerry, MK.
• Cameron-Smith, D
• [+] on PubMed
• Farnfield, MM.
• Carey, KA.
• Gran, P
• Trenerry, MK.
• Cameron-Smith, D

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Nutrients 2009, 1(2), 263-275; doi:10.3390/nu1020263

Article

Whey Protein Ingestion Activates mTOR-dependent Signalling after Resistance Exercise in Young Men: A Double-Blinded Randomized Controlled Trial

Michelle M. Farnfield, Kate A. Carey, Petra Gran, Marissa K. Trenerry and David Cameron-Smith*

Molecular Nutrition Unit, Deakin University, Burwood, Victoria, 3125, Australia

* Author to whom correspondence should be addressed.

Received: 10 November 2009 / Accepted: 4 December 2009 / Published: 14 December 2009

Download PDF Full-Text [286 KB, uploaded 15 December 2009 08:19 CET]

Abstract: The effect of resistance exercise with the ingestion of supplementary protein on the activation of the mTOR cascade, in human skeletal muscle has not been fully elucidated. In this study, the impact of a single bout of resistance exercise, immediately followed by a single dose of whey protein isolate (WPI) or placebo supplement, on the activation of mTOR signalling was analyzed. Young untrained men completed a maximal single-legged knee extension exercise bout and were randomized to ingest either WPI supplement (n = 7) or the placebo (n = 7). Muscle biopsies were taken from the vastus lateralis before, and 2, 4 and 24 hr post-exercise. WPI or placebo ingestion consumed immediately post-exercise had no impact on the phosphorylation of Akt (Ser⁴⁷³). However, WPI significantly enhanced phosphorylation of mTOR (Ser²⁴⁴⁸), 4E-BP1 (Thr^37/46) and p70^S6K (Thr³⁸⁹) at 2 hr post-exercise. This study demonstrates that a single dose of WPI, when consumed in modest quantities, taken immediately after resistance exercise elicits an acute and transient activation of translation initiation within the exercised skeletal muscle.

Keywords: leucine; BCAA; p70^S6K; 4E-BP1; resistance exercise

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