Next Article in Journal
Lightweight Design Solutions in the Automotive Field: Environmental Modelling Based on Fuel Reduction Value Applied to Diesel Turbocharged Vehicles
Next Article in Special Issue
Fungal Pretreatment of Willow Sawdust with Abortiporus biennis for Anaerobic Digestion: Impact of an External Nitrogen Source
Previous Article in Journal
A Pro-Environmental Reasoned Action Model for Measuring Citizens’ Intentions regarding Ecolabel Product Usage
Previous Article in Special Issue
Model of Chinese Household Kitchen Waste Separation Behavior: A Case Study in Beijing City
Article Menu

Export Article

Open AccessArticle
Sustainability 2016, 8(11), 1168; doi:10.3390/su8111168

Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation

1
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice 5700, South Africa
2
Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Alice 5700, South Africa
*
Author to whom correspondence should be addressed.
Academic Editor: Dimitrios Komilis
Received: 24 August 2016 / Revised: 7 November 2016 / Accepted: 9 November 2016 / Published: 11 November 2016
(This article belongs to the Special Issue Sustainability in Solid Waste Management)
View Full-Text   |   Download PDF [1400 KB, uploaded 11 November 2016]   |  

Abstract

This paper reports on the optimization of culture conditions for cellulase and xylanase production by bacterial isolate from lignocellulosic biomass. The bacterial isolate was screened for cellulase and xylanase production on carboxyl methyl cellulose (CMC) and birch wood xylan as substrates, respectively. One bacterial isolate showing the highest halo zone diameter (isolate PLY1) was selected for detailed studies. The analysis of the 16S ribosomal ribonucleic acid (rRNA) gene nucleotide sequence of PLY1 revealed it to have 98% similarity to Micrococcus luteus strain Fse9 and the sequence was deposited in the GenBank as Micrococcus luteus strain SAMRC-UFH3 with accession number KU171371. Cellulase production was achieved in the presence of CMC (1% w/v) under an incubation temperature of 25 °C (198 U/mL), pH 5 (173 U/mL), agitation speed 50 rpm (173 U/mL) and incubation period of 96 h (102 U/mL). Xylanase was produced maximally when birch wood xylan (1% w/v) was used as the substrate at 25 °C (1007 U/mL), pH 10 (2487 U/mL), 200 rpm (1814 U/mL), and under an incubation period of 84 h (1296 U/mL). Our findings showed that Micrococcus sp. SAMRC-UFH3 appears to be a potentially important candidate for lignocellulosic waste degradation and other relevant industrial applications. View Full-Text
Keywords: lignocellulosic biomass; Micrococcus sp. SAMRC-UFH3; culture conditions; cellulase; Xylanase lignocellulosic biomass; Micrococcus sp. SAMRC-UFH3; culture conditions; cellulase; Xylanase
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Mmango-Kaseke, Z.; Okaiyeto, K.; Nwodo, U.U.; Mabinya, L.V.; Okoh, A.I. Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation. Sustainability 2016, 8, 1168.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sustainability EISSN 2071-1050 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top