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Viruses 2017, 9(1), 9; doi:10.3390/v9010009

Effective Detection of Porcine Cytomegalovirus Using Non-Invasively Taken Samples from Piglets

1
Robert Koch Institute, 13353 Berlin, Germany
2
Aachen Minipigs, 52525 Heinsberg, Germany
*
Authors to whom correspondence should be addressed.
Academic Editors: Linda Dixon and Simon Graham
Received: 20 October 2016 / Revised: 29 December 2016 / Accepted: 3 January 2017 / Published: 12 January 2017
(This article belongs to the Special Issue Porcine Viruses)
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Abstract

Shortage of human organs forced the development of xenotransplantation using cells, tissues, and organs from pigs. Xenotransplantation may be associated with the transmission of porcine zoonotic microorganisms, among them the porcine cytomegalovirus (PCMV). To prevent virus transmission, pigs have to be screened using sensitive methods. In order to perform regular follow-ups and further breeding of the animals, samples for testing should be collected by low-invasive or non-invasive methods. Sera, ear biopsies, as well as oral and anal swabs were collected from ten 10-day-old Aachen minipigs (AaMP) and tested for PCMV using sensitive nested polymerase chain reaction (PCR) as well as uniplex and duplex real-time PCR. Porcine cytomegalovirus DNA was detected most frequently in oral and anal swabs. Comparison of duplex and uniplex real-time PCR systems for PCMV detection demonstrated a lower sensitivity of duplex real-time PCR when the copy numbers of the target genes were low (less 200). Therefore, to increase the efficacy of PCMV detection in piglets, early testing of oral and anal swabs by uniplex real-time PCR is recommended. View Full-Text
Keywords: Aachen minipigs (AaMP); non-invasive sampling; porcine cytomegalovirus (PCMV); infection; detection; sensitivity; nested PCR; real-time PCR Aachen minipigs (AaMP); non-invasive sampling; porcine cytomegalovirus (PCMV); infection; detection; sensitivity; nested PCR; real-time PCR
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Morozov, V.A.; Heinrichs, G.; Denner, J. Effective Detection of Porcine Cytomegalovirus Using Non-Invasively Taken Samples from Piglets. Viruses 2017, 9, 9.

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