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Viruses 2016, 8(12), 327; doi:10.3390/v8120327

Characterization of an Immunodominant Epitope in the Endodomain of the Coronavirus Membrane Protein

1
State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
2
Molecular Biology (Gembloux Agro-Bio Tech), University of Liège (ULg), Liège 4000, Belgium
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editors: Linda Dixon and Simon Graham
Received: 24 October 2016 / Revised: 26 November 2016 / Accepted: 6 December 2016 / Published: 10 December 2016
(This article belongs to the Special Issue Porcine Viruses)
View Full-Text   |   Download PDF [38236 KB, uploaded 12 December 2016]   |  

Abstract

The coronavirus membrane (M) protein acts as a dominant immunogen and is a major player in virus assembly. In this study, we prepared two monoclonal antibodies (mAbs; 1C3 and 4C7) directed against the transmissible gastroenteritis virus (TGEV) M protein. The 1C3 and 4C7 mAbs both reacted with the native TGEV M protein in western blotting and immunofluorescence (IFA) assays. Two linear epitopes, 243YSTEART249 (1C3) and 243YSTEARTDNLSEQEKLLHMV262 (4C7), were identified in the endodomain of the TGEV M protein. The 1C3 mAb can be used for the detection of the TGEV M protein in different assays. An IFA method for the detection of TGEV M protein was optimized using mAb 1C3. Furthermore, the ability of the epitope identified in this study to stimulate antibody production was also evaluated. An immunodominant epitope in the TGEV membrane protein endodomain was identified. The results of this study have implications for further research on TGEV replication. View Full-Text
Keywords: immunodominant epitope; coronavirus; membrane protein; endodomain immunodominant epitope; coronavirus; membrane protein; endodomain
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Dong, H.; Zhang, X.; Shi, H.; Chen, J.; Shi, D.; Zhu, Y.; Feng, L. Characterization of an Immunodominant Epitope in the Endodomain of the Coronavirus Membrane Protein. Viruses 2016, 8, 327.

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