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Viruses 2017, 9(1), 19; doi:10.3390/v9010019

Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China
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Academic Editors: Linda Dixon and Simon Graham
Received: 6 December 2016 / Revised: 13 January 2017 / Accepted: 17 January 2017 / Published: 21 January 2017
(This article belongs to the Special Issue Porcine Viruses)
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Abstract

Porcine epidemic diarrhea virus (PEDV), which is the causative agent of porcine epidemic diarrhea in China and other countries, is responsible for serious economic losses in the pork industry. Inactivated PEDV vaccine plays a key role in controlling the prevalence of PEDV. However, consistently low viral titers are obtained during the propagation of PEDV in vitro; this represents a challenge to molecular analyses of the virus and vaccine development. In this study, we successfully isolated a PEDV isolate (strain NJ) from clinical samples collected during a recent outbreak of diarrhea in piglets in China, using porcine intestinal epithelial cells (IEC). We found that the isolate was better adapted to growth in IECs than in Vero cells, and the titer of the IEC cultures was 104.5 TCID50/0.1 mL at passage 45. Mutations in the S protein increased with the viral passage and the mutations tended towards attenuation. Viral challenge showed that the survival of IEC-adapted cultures was higher at the 45th passage than at the 5th passage. The use of IECs to isolate and propagate PEDV provides an effective approach for laboratory-based diagnosis of PEDV, as well as studies of the epidemiological characteristics and molecular biology of this virus. View Full-Text
Keywords: porcine epidemic diarrhea virus (PEDV) NJ strain; porcine intestinal epithelial cells; isolation and identification porcine epidemic diarrhea virus (PEDV) NJ strain; porcine intestinal epithelial cells; isolation and identification
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MDPI and ACS Style

Shi, W.; Jia, S.; Zhao, H.; Yin, J.; Wang, X.; Yu, M.; Ma, S.; Wu, Y.; Chen, Y.; Fan, W.; Xu, Y.; Li, Y. Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells. Viruses 2017, 9, 19.

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