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Viruses 2017, 9(2), 31; doi:10.3390/v9020031

Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants

1
Central Veterinary Laboratory, Avenue Wangata, P.O. Box 8842, Kinshasa I, Democratic Republic of Congo
2
Research Unit of Epidemiology and Risk Analysis Applied to Veterinary (UREAR-Ulg), Fundamental and Applied Research for Animals & Health, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium
3
Animal Production and Health Laboratory, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, Austria
4
Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
5
Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 ONF, UK
6
Centro de Investigaciόn en Sanidad Animal (CISA), INIA, Valdeolmos, 28130 Madrid, Spain
7
Oak Ridge Institute for Science and Education (ORISE) CDC Fellowship Program, Oak Ridge, TN 37830, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Eric O. Freed
Received: 19 November 2016 / Revised: 8 February 2017 / Accepted: 10 February 2017 / Published: 18 February 2017
(This article belongs to the Special Issue Porcine Viruses)
View Full-Text   |   Download PDF [3119 KB, uploaded 18 February 2017]   |  

Abstract

African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were initially eradicated—except for Sardinia—followed by re‑introduction into Europe in 2007. In this study of ASF within the Democratic Republic of the Congo, 62 domestic pig samples, collected between 2005–2012, were examined for viral DNA and sequencing at multiple loci: C-terminus of the B646L gene (p72 protein), central hypervariable region (CVR) of the B602L gene, and the E183L gene (p54 protein). Phylogenetic analyses identified three circulating genotypes: I (64.5% of samples), IX (32.3%), and XIV (3.2%). This is the first evidence of genotypes IX and XIV within this country. Examination of the CVR revealed high levels of intra-genotypic variation, with 19 identified variants. View Full-Text
Keywords: African swine fever virus; outbreaks; Democratic Republic of Congo; swine; genotypes; molecular epidemiology; p72 gene; p54 gene; CVR African swine fever virus; outbreaks; Democratic Republic of Congo; swine; genotypes; molecular epidemiology; p72 gene; p54 gene; CVR
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MDPI and ACS Style

Mulumba–Mfumu, L.K.; Achenbach, J.E.; Mauldin, M.R.; Dixon, L.K.; Tshilenge, C.G.; Thiry, E.; Moreno, N.; Blanco, E.; Saegerman, C.; Lamien, C.E.; Diallo, A. Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants. Viruses 2017, 9, 31.

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