Development of Neutralization Assay Using an eGFP Chikungunya Virus
AbstractChikungunya virus (CHIKV), a member of the Alphavirus genus, is an important human emerging/re-emerging pathogen. Currently, there are no effective antiviral drugs or vaccines against CHIKV infection. Herein, we construct an infectious clone of CHIKV and an eGFP reporter CHIKV (eGFP-CHIKV) with an isolated strain (assigned to Asian lineage) from CHIKV-infected patients. The eGFP-CHIKV reporter virus allows for direct visualization of viral replication through the levels of eGFP expression. Using a known CHIKV inhibitor, ribavirin, we confirmed that the eGFP-CHIKV reporter virus could be used to identify inhibitors against CHIKV. Importantly, we developed a novel and reliable eGFP-CHIKV reporter virus-based neutralization assay that could be used for rapid screening neutralizing antibodies against CHIKV. View Full-Text
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Deng, C.-L.; Liu, S.-Q.; Zhou, D.-G.; Xu, L.-L.; Li, X.-D.; Zhang, P.-T.; Li, P.-H.; Ye, H.-Q.; Wei, H.-P.; Yuan, Z.-M.; Qin, C.-F.; Zhang, B. Development of Neutralization Assay Using an eGFP Chikungunya Virus. Viruses 2016, 8, 181.
Deng C-L, Liu S-Q, Zhou D-G, Xu L-L, Li X-D, Zhang P-T, Li P-H, Ye H-Q, Wei H-P, Yuan Z-M, Qin C-F, Zhang B. Development of Neutralization Assay Using an eGFP Chikungunya Virus. Viruses. 2016; 8(7):181.Chicago/Turabian Style
Deng, Cheng-Lin; Liu, Si-Qing; Zhou, Dong-Gen; Xu, Lin-Lin; Li, Xiao-Dan; Zhang, Pan-Tao; Li, Peng-Hui; Ye, Han-Qing; Wei, Hong-Ping; Yuan, Zhi-Ming; Qin, Cheng-Feng; Zhang, Bo. 2016. "Development of Neutralization Assay Using an eGFP Chikungunya Virus." Viruses 8, no. 7: 181.
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