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Viruses 2016, 8(7), 181; doi:10.3390/v8070181

Development of Neutralization Assay Using an eGFP Chikungunya Virus

1
Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
2
Ningbo International Travel Healthcare Center, Ningbo 315012, China
3
School of Medicine, Hunan Normal University, Changsha 410000, China
4
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Luis Martinez-Sobrido
Received: 2 March 2016 / Revised: 31 May 2016 / Accepted: 23 June 2016 / Published: 28 June 2016
(This article belongs to the Special Issue Replication-Competent Reporter-Expressing Viruses)
View Full-Text   |   Download PDF [20768 KB, uploaded 28 June 2016]   |  

Abstract

Chikungunya virus (CHIKV), a member of the Alphavirus genus, is an important human emerging/re-emerging pathogen. Currently, there are no effective antiviral drugs or vaccines against CHIKV infection. Herein, we construct an infectious clone of CHIKV and an eGFP reporter CHIKV (eGFP-CHIKV) with an isolated strain (assigned to Asian lineage) from CHIKV-infected patients. The eGFP-CHIKV reporter virus allows for direct visualization of viral replication through the levels of eGFP expression. Using a known CHIKV inhibitor, ribavirin, we confirmed that the eGFP-CHIKV reporter virus could be used to identify inhibitors against CHIKV. Importantly, we developed a novel and reliable eGFP-CHIKV reporter virus-based neutralization assay that could be used for rapid screening neutralizing antibodies against CHIKV. View Full-Text
Keywords: Chikungunya virus; reporter virus; antiviral; neutralization antibody Chikungunya virus; reporter virus; antiviral; neutralization antibody
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Deng, C.-L.; Liu, S.-Q.; Zhou, D.-G.; Xu, L.-L.; Li, X.-D.; Zhang, P.-T.; Li, P.-H.; Ye, H.-Q.; Wei, H.-P.; Yuan, Z.-M.; Qin, C.-F.; Zhang, B. Development of Neutralization Assay Using an eGFP Chikungunya Virus. Viruses 2016, 8, 181.

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