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Viruses 2016, 8(7), 179; doi:10.3390/v8070179

Replication-Competent Influenza A Viruses Expressing Reporter Genes

Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editor: Eric O. Freed
Received: 17 April 2016 / Revised: 16 June 2016 / Accepted: 19 June 2016 / Published: 23 June 2016
(This article belongs to the Special Issue Replication-Competent Reporter-Expressing Viruses)
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Abstract

Influenza A viruses (IAV) cause annual seasonal human respiratory disease epidemics. In addition, IAV have been implicated in occasional pandemics with inordinate health and economic consequences. Studying IAV, in vitro or in vivo, requires the use of laborious secondary methodologies to identify virus-infected cells. To circumvent this requirement, replication-competent IAV expressing an easily traceable reporter protein can be used. Here we discuss the development and applications of recombinant replication-competent IAV harboring diverse fluorescent or bioluminescent reporter genes in different locations of the viral genome. These viruses have been employed for in vitro and in vivo studies, such as the screening of neutralizing antibodies or antiviral compounds, the identification of host factors involved in viral replication, cell tropism, the development of vaccines, or the assessment of viral infection dynamics. In summary, reporter-expressing, replicating-competent IAV represent a powerful tool for the study of IAV both in vitro and in vivo. View Full-Text
Keywords: recombinant influenza A virus; plasmid-based reverse genetics; virus rescue approaches; reporter genes; fluorescence; luminescence; replicating-competent reporter-expressing influenza A virus recombinant influenza A virus; plasmid-based reverse genetics; virus rescue approaches; reporter genes; fluorescence; luminescence; replicating-competent reporter-expressing influenza A virus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Breen, M.; Nogales, A.; Baker, S.F.; Martínez-Sobrido, L. Replication-Competent Influenza A Viruses Expressing Reporter Genes. Viruses 2016, 8, 179.

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