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Viruses 2015, 7(3), 915-938; doi:10.3390/v7030915

HSV-1 gM and the gK/pUL20 Complex Are Important for the Localization of gD and gH/L to Viral Assembly Sites

Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
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Author to whom correspondence should be addressed.
Academic Editors: Marc Johnson and Shan-Lu Liu
Received: 22 December 2014 / Revised: 6 February 2015 / Accepted: 22 February 2015 / Published: 4 March 2015
(This article belongs to the Special Issue Viral Glycoprotein Incorporation)
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Abstract

Herpes simplex virus-1 (HSV-1), like all herpesviruses, is a large complex DNA virus containing up to 16 different viral membrane proteins in its envelope. The assembly of HSV-1 particles occurs by budding/wrapping at intracellular membranes producing infectious virions contained within the lumen of cytoplasmic membrane-bound compartments that are then released by secretion. To ensure incorporation of all viral membrane proteins into the envelope, they need to be localized to the appropriate intracellular membranes either via the endocytic pathway or by direct targeting to assembly sites from the biosynthetic secretory pathway. Many HSV-1 envelope proteins encode targeting motifs that direct their endocytosis and targeting, while others do not, including the essential entry proteins gD and the gH/gL complex, and so it has been unclear how these envelope proteins reach the appropriate assembly compartments. We now show that efficient endocytosis of gD and gH/gL and their incorporation into mature virions relies upon the presence of the HSV-1 envelope proteins gM and the gK/pUL20 complex. Our data demonstrate both redundant and synergistic roles for gM and gK/pUL20 in controlling the targeting of gD and gH/L to the appropriate intracellular virus assembly compartments. View Full-Text
Keywords: HSV-1; glycoprotein M; glycoprotein K; UL20; glycoprotein D; glycoprotein H; virus assembly HSV-1; glycoprotein M; glycoprotein K; UL20; glycoprotein D; glycoprotein H; virus assembly
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lau, S.-Y.K.; Crump, C.M. HSV-1 gM and the gK/pUL20 Complex Are Important for the Localization of gD and gH/L to Viral Assembly Sites. Viruses 2015, 7, 915-938.

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