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Viruses 2014, 6(5), 2028-2037; doi:10.3390/v6052028
Short Communication

Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals

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Received: 5 December 2013 / Revised: 16 April 2014 / Accepted: 20 April 2014 / Published: 6 May 2014
(This article belongs to the Special Issue Hantaviruses)
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Abstract: We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species.
Keywords: Andes virus; Chile; ELISA; strip immunoblot assay; hantavirus Andes virus; Chile; ELISA; strip immunoblot assay; hantavirus
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Cautivo, K.; Schountz, T.; Acuña-Retamar, M.; Ferrés, M.; Torres-Pérez, F. Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals. Viruses 2014, 6, 2028-2037.

AMA Style

Cautivo K, Schountz T, Acuña-Retamar M, Ferrés M, Torres-Pérez F. Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals. Viruses. 2014; 6(5):2028-2037.

Chicago/Turabian Style

Cautivo, Karla; Schountz, Tony; Acuña-Retamar, Mariana; Ferrés, Marcela; Torres-Pérez, Fernando. 2014. "Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals." Viruses 6, no. 5: 2028-2037.


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