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Viruses 2014, 6(5), 2028-2037; doi:10.3390/v6052028

Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals

1
Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso 2373223, Chile
2
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
3
Facultad de Cs Veterinarias y Pecuarias, Universidad de Chile, Santiago 8820808, Chile
4
Laboratorio de Infectología y Virología Molecular, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago 8330024, Chile
*
Author to whom correspondence should be addressed.
Received: 5 December 2013 / Revised: 16 April 2014 / Accepted: 20 April 2014 / Published: 6 May 2014
(This article belongs to the Special Issue Hantaviruses)
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Abstract

We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species. View Full-Text
Keywords: Andes virus; Chile; ELISA; strip immunoblot assay; hantavirus Andes virus; Chile; ELISA; strip immunoblot assay; hantavirus
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MDPI and ACS Style

Cautivo, K.; Schountz, T.; Acuña-Retamar, M.; Ferrés, M.; Torres-Pérez, F. Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals. Viruses 2014, 6, 2028-2037.

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