Viruses 2014, 6(4), 1525-1539; doi:10.3390/v6041525
Article

Comprehensive Characterization of Serum MicroRNA Profile in Response to the Emerging Avian Influenza A (H7N9) Virus Infection in Humans

1,†email, 1,†email, 2,†email, 2email, 2email, 2email, 1email, 1,* email and 1,* email
Received: 31 December 2013; in revised form: 13 February 2014 / Accepted: 18 March 2014 / Published: 2 April 2014
(This article belongs to the Special Issue Viruses and miRNAs)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A novel avian-origin influenza A (H7N9) virus recently occurred in China and caused 137 human infection cases with a 32.8% mortality rate. Although various detection procedures have been developed, the pathogenesis of this emerging virus in humans remains largely unknown. In this study, we characterized serum microRNA (miRNA) profile in response to H7N9 virus infection using TaqMan Low Density Arrays. Upon infection, a total of 395 miRNAs were expressed in the serum pool of patients, far beyond the 221 in healthy controls. Among the 187 commonly expressed miRNAs, 146 were up-regulated and only 7 down-regulated in patients. Further analysis by quantitative RT-PCR revealed that the serum levels of miR-17, miR-20a, miR-106a and miR-376c were significantly elevated in patients compared with healthy individuals (p < 0.05). Receiver operating characteristic (ROC) curves were constructed to show that each miRNA could discriminate H7N9 patients from controls with area under the curve (AUC) values ranging from 0.622 to 0.898, whereas a combination of miR-17, miR-20a, miR-106a and miR-376c obtained a higher discriminating ability with an AUC value of 0.96. Our findings unravel the significant alterations in serum miRNA expression following virus infection and manifest great potential of circulating miRNAs for the diagnosis of viral diseases.
Keywords: H7N9 virus; avian influenza; serum microRNA; expression profile
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MDPI and ACS Style

Zhu, Z.; Qi, Y.; Ge, A.; Zhu, Y.; Xu, K.; Ji, H.; Shi, Z.; Cui, L.; Zhou, M. Comprehensive Characterization of Serum MicroRNA Profile in Response to the Emerging Avian Influenza A (H7N9) Virus Infection in Humans. Viruses 2014, 6, 1525-1539.

AMA Style

Zhu Z, Qi Y, Ge A, Zhu Y, Xu K, Ji H, Shi Z, Cui L, Zhou M. Comprehensive Characterization of Serum MicroRNA Profile in Response to the Emerging Avian Influenza A (H7N9) Virus Infection in Humans. Viruses. 2014; 6(4):1525-1539.

Chicago/Turabian Style

Zhu, Zheng; Qi, Yuhua; Ge, Aihua; Zhu, Yefei; Xu, Ke; Ji, Hong; Shi, Zhiyang; Cui, Lunbiao; Zhou, Minghao. 2014. "Comprehensive Characterization of Serum MicroRNA Profile in Response to the Emerging Avian Influenza A (H7N9) Virus Infection in Humans." Viruses 6, no. 4: 1525-1539.


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