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Viruses 2014, 6(11), 4346-4357; doi:10.3390/v6114346

Genetic Characterization of Goutanap Virus, a Novel Virus Related to Negeviruses, Cileviruses and Higreviruses

1
Institute of Virology, Medical Centre, University of Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany
2
Estación de Biología Tropical Los Tuxtlas, Instituto de Biología, Universidad Nacional Autónoma de México, Apdo 176, San Andres Tuxtla, Veracruz, Mexico
3
Department of Environmental Sciences and Program in Population Biology, Ecology and Evolution, Emory University, Atlanta, GA 30322, USA
4
Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA 30322, USA
These authors contributed equally to this work.
Present address: Helmholtz Centre for Environmental Research
*
Author to whom correspondence should be addressed.
Received: 1 August 2014 / Revised: 28 October 2014 / Accepted: 3 November 2014 / Published: 12 November 2014
(This article belongs to the Special Issue Impact of the Insect Microbiome on Arbovirus Transmission)
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Abstract

Pools of mosquitoes collected in Côte d’Ivoire and Mexico were tested for cytopathic effects on the mosquito cell line C6/36. Seven pools induced strong cytopathic effects after one to five days post infection and were further investigated by deep sequencing. The genomes of six virus isolates from Côte d’Ivoire showed pairwise nucleotide identities of ~99% among each other and of 56%–60% to Dezidougou virus and Wallerfield virus, two insect-specific viruses belonging to the proposed new taxon Negevirus. The novel virus was tentatively named Goutanap virus. The isolate derived from the Mexican mosquitoes showed 95% pairwise identity to Piura virus and was suggested to be a strain of Piura virus, named C6.7-MX-2008. Phylogenetic inferences based on a concatenated alignment of the methyltransferase, helicase, and RNA-dependent RNA polymerase domains showed that the new taxon Negevirus formed two monophyletic clades, named Nelorpivirus and Sandewavirus after the viruses grouping in these clades. Branch lengths separating these clades were equivalent to those of the related genera Cilevirus, Higrevirus and Blunervirus, as well as to those within the family Virgaviridae. Genetic distances and phylogenetic analyses suggest that Nelorpivirus and Sandewavirus might form taxonomic groups on genus level that may define alone or together with Cilevirus, Higrevirus and Blunervirus a viral family. View Full-Text
Keywords: negevirus; insect-specific viruses; mosquito; next generation sequencing; virus taxonomy negevirus; insect-specific viruses; mosquito; next generation sequencing; virus taxonomy
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MDPI and ACS Style

Kallies, R.; Kopp, A.; Zirkel, F.; Estrada, A.; Gillespie, T.R.; Drosten, C.; Junglen, S. Genetic Characterization of Goutanap Virus, a Novel Virus Related to Negeviruses, Cileviruses and Higreviruses. Viruses 2014, 6, 4346-4357.

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