Viruses 2013, 5(11), 2679-2689; doi:10.3390/v5112679
Brief Report

Detection of Coronaviruses in Bats of Various Species in Italy

1,†,* email, 1,†email, 2email, 3email, 1email and 1email
Received: 27 September 2013; in revised form: 25 October 2013 / Accepted: 28 October 2013 / Published: 31 October 2013
(This article belongs to the Special Issue Viruses and Bats)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Bats are natural reservoirs for many mammalian coronaviruses, which have received renewed interest after the discovery of the severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS) CoV in humans. This study describes the identification and molecular characterization of alphacoronaviruses and betacoronaviruses in bats in Italy, from 2010 to 2012. Sixty-nine faecal samples and 126 carcasses were tested using pan-coronavirus RT-PCR. Coronavirus RNAs were detected in seven faecal samples and nine carcasses. A phylogenetic analysis of RNA-dependent RNA polymerase sequence fragments aided in identifying two alphacoronaviruses from Kuhl’s pipistrelle (Pipistrellus kuhlii), three clade 2b betacoronaviruses from lesser horseshoe bats (Rhinolophus hipposideros), and 10 clade 2c betacoronaviruses from Kuhl’s pipistrelle, common noctule (Nyctalus noctula), and Savi’s pipistrelle (Hypsugo savii). This study fills a substantive gap in the knowledge on bat-CoV ecology in Italy, and extends the current knowledge on clade 2c betacoronaviruses with new sequences obtained from bats that have not been previously described as hosts of these viruses.
Keywords: coronavirus; bats; Italy; molecular characterization; phylogenetic analysis
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MDPI and ACS Style

Lelli, D.; Papetti, A.; Sabelli, C.; Rosti, E.; Moreno, A.; Boniotti, M.B. Detection of Coronaviruses in Bats of Various Species in Italy. Viruses 2013, 5, 2679-2689.

AMA Style

Lelli D, Papetti A, Sabelli C, Rosti E, Moreno A, Boniotti MB. Detection of Coronaviruses in Bats of Various Species in Italy. Viruses. 2013; 5(11):2679-2689.

Chicago/Turabian Style

Lelli, Davide; Papetti, Alice; Sabelli, Cristiano; Rosti, Enrica; Moreno, Ana; Boniotti, Maria B. 2013. "Detection of Coronaviruses in Bats of Various Species in Italy." Viruses 5, no. 11: 2679-2689.

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