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Detection of Coronaviruses in Bats of Various Species in Italy
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Via Bianchi 9, Brescia 25124, Italy
IZO s.r.l, Via S. Zeno 99/A, Brescia 25124, Italy
Centro Fauna Selvatica "Il Pettirosso", Via Nonantolana 1217, Modena 41122, Italy
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 27 September 2013; in revised form: 25 October 2013 / Accepted: 28 October 2013 / Published: 31 October 2013
Abstract: Bats are natural reservoirs for many mammalian coronaviruses, which have received renewed interest after the discovery of the severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS) CoV in humans. This study describes the identification and molecular characterization of alphacoronaviruses and betacoronaviruses in bats in Italy, from 2010 to 2012. Sixty-nine faecal samples and 126 carcasses were tested using pan-coronavirus RT-PCR. Coronavirus RNAs were detected in seven faecal samples and nine carcasses. A phylogenetic analysis of RNA-dependent RNA polymerase sequence fragments aided in identifying two alphacoronaviruses from Kuhl’s pipistrelle (Pipistrellus kuhlii), three clade 2b betacoronaviruses from lesser horseshoe bats (Rhinolophus hipposideros), and 10 clade 2c betacoronaviruses from Kuhl’s pipistrelle, common noctule (Nyctalus noctula), and Savi’s pipistrelle (Hypsugo savii). This study fills a substantive gap in the knowledge on bat-CoV ecology in Italy, and extends the current knowledge on clade 2c betacoronaviruses with new sequences obtained from bats that have not been previously described as hosts of these viruses.
Keywords: coronavirus; bats; Italy; molecular characterization; phylogenetic analysis
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MDPI and ACS Style
Lelli, D.; Papetti, A.; Sabelli, C.; Rosti, E.; Moreno, A.; Boniotti, M.B. Detection of Coronaviruses in Bats of Various Species in Italy. Viruses 2013, 5, 2679-2689.
Lelli D, Papetti A, Sabelli C, Rosti E, Moreno A, Boniotti MB. Detection of Coronaviruses in Bats of Various Species in Italy. Viruses. 2013; 5(11):2679-2689.
Lelli, Davide; Papetti, Alice; Sabelli, Cristiano; Rosti, Enrica; Moreno, Ana; Boniotti, Maria B. 2013. "Detection of Coronaviruses in Bats of Various Species in Italy." Viruses 5, no. 11: 2679-2689.