Next Article in Journal / Special Issue
Interplay between Interferon-Mediated Innate Immunity and Porcine Reproductive and Respiratory Syndrome Virus
Previous Article in Journal
Innate Immunity Evasion by Dengue Virus
Previous Article in Special Issue
The Coronavirus E Protein: Assembly and Beyond
Article Menu

Export Article

Open AccessCommunication
Viruses 2012, 4(3), 414-423; doi:10.3390/v4030414

Folding and Oligomerization of the gp2b/gp3/gp4 Spike Proteins of Equine Arteritis Virus in Vitro

Department of Immunology and Molecular Biology, Veterinary Faculty, Free University, Philippstraße 13, Berlin 10115, Germany
*
Author to whom correspondence should be addressed.
Received: 5 January 2012 / Revised: 13 March 2012 / Accepted: 18 March 2012 / Published: 22 March 2012
(This article belongs to the Special Issue Animal Arteriviruses and Coronaviruses)
View Full-Text   |   Download PDF [1470 KB, uploaded 12 May 2015]   |  

Abstract

Equine arteritis virus (EAV) is a small, positive-stranded RNA virus. The glycoproteins gp2b, gp3 and gp4 form a heterotrimer in the viral envelope, which is required for cell entry of EAV. We describe expression of the ectodomains of the proteins in E. coli and their refolding from inclusion bodies. After extraction of inclusion bodies and dialysis, Gst-, but not His-tagged proteins, refold into a soluble conformation. However, when dialyzed together with Gst-gp3 or with Gst-gp4, His-gp2b and His-gp4 remain soluble and oligomers are obtained by affinity-chromatography. Thus, folding and oligomerization of gp2b, gp3 and gp4 in vitro are interdependent processes.
Keywords: equine arteritis virus; glycoproteins; gp2b; gp3; gp4; protein folding, oligomerization equine arteritis virus; glycoproteins; gp2b; gp3; gp4; protein folding, oligomerization
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kabatek, A.; Veit, M. Folding and Oligomerization of the gp2b/gp3/gp4 Spike Proteins of Equine Arteritis Virus in Vitro. Viruses 2012, 4, 414-423.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top