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Sharka: The Past, The Present and The Future
Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, CZ-612 42, Czech Republic
Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic
Department of Fruit Growing, Faculty of Horticulture, Mendel University in Brno, Valticka 337, CZ-691 44 Lednice, Czech Republic
* Author to whom correspondence should be addressed.
Received: 15 October 2012; in revised form: 25 October 2012 / Accepted: 30 October 2012 / Published: 7 November 2012
Abstract: Members the Potyviridae family belong to a group of plant viruses that are causing devastating plant diseases with a significant impact on agronomy and economics. Plum pox virus (PPV), as a causative agent of sharka disease, is widely discussed. The understanding of the molecular biology of potyviruses including PPV and the function of individual proteins as products of genome expression are quite necessary for the proposal the new antiviral strategies. This review brings to view the members of Potyviridae family with respect to plum pox virus. The genome of potyviruses is discussed with respect to protein products of its expression and their function. Plum pox virus distribution, genome organization, transmission and biochemical changes in infected plants are introduced. In addition, techniques used in PPV detection are accentuated and discussed, especially with respect to new modern techniques of nucleic acids isolation, based on the nanotechnological approach. Finally, perspectives on the future of possibilities for nanotechnology application in PPV determination/identification are outlined.
Keywords: viruses; plant; Potyviridae; plum pox virus; sharka; polymerase chain reaction; immune detection; ELISA; nanotechnology
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Sochor, J.; Babula, P.; Adam, V.; Krska, B.; Kizek, R. Sharka: The Past, The Present and The Future. Viruses 2012, 4, 2853-2901.
Sochor J, Babula P, Adam V, Krska B, Kizek R. Sharka: The Past, The Present and The Future. Viruses. 2012; 4(11):2853-2901.
Sochor, Jiri; Babula, Petr; Adam, Vojtech; Krska, Boris; Kizek, Rene. 2012. "Sharka: The Past, The Present and The Future." Viruses 4, no. 11: 2853-2901.