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Viruses 2009, 1(3), 1295-1324; doi:10.3390/v1031295

Viral Hybrid Vectors for Somatic Integration - Are They the Better Solution?

Max von Pettenkofer-Institut, Department of Virology, Ludwig-Maximilians-Universität Munich, Pettenkoferstr. 9A, 80336 Munich, Germany
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Received: 30 September 2009 / Revised: 4 December 2009 / Accepted: 10 December 2009 / Published: 15 December 2009
(This article belongs to the Special Issue Novel Viral Vector Systems for Gene Therapy)
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Abstract

The turbulent history of clinical trials in viral gene therapy has taught us important lessons about vector design and safety issues. Much effort was spent on analyzing genotoxicity after somatic integration of therapeutic DNA into the host genome. Based on these findings major improvements in vector design including the development of viral hybrid vectors for somatic integration have been achieved. This review provides a state-of-the-art overview of available hybrid vectors utilizing viruses for high transduction efficiencies in concert with various integration machineries for random and targeted integration patterns. It discusses advantages but also limitations of each vector system.
Keywords: hybrid vector; somatic integration; adenovirus; retrovirus; adeno-associated virus; herpes simplex virus; targeted integration; Sleeping Beauty transposase; PhiC31 integrase; zinc-finger nuclease hybrid vector; somatic integration; adenovirus; retrovirus; adeno-associated virus; herpes simplex virus; targeted integration; Sleeping Beauty transposase; PhiC31 integrase; zinc-finger nuclease
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Müther, N.; Noske, N.; Ehrhardt, A. Viral Hybrid Vectors for Somatic Integration - Are They the Better Solution? Viruses 2009, 1, 1295-1324.

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