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Viral Hybrid Vectors for Somatic Integration - Are They the Better Solution?
Max von Pettenkofer-Institut, Department of Virology, Ludwig-Maximilians-Universität Munich, Pettenkoferstr. 9A, 80336 Munich, Germany
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Received: 30 September 2009; in revised form: 4 December 2009 / Accepted: 10 December 2009 / Published: 15 December 2009
Abstract: The turbulent history of clinical trials in viral gene therapy has taught us important lessons about vector design and safety issues. Much effort was spent on analyzing genotoxicity after somatic integration of therapeutic DNA into the host genome. Based on these findings major improvements in vector design including the development of viral hybrid vectors for somatic integration have been achieved. This review provides a state-of-the-art overview of available hybrid vectors utilizing viruses for high transduction efficiencies in concert with various integration machineries for random and targeted integration patterns. It discusses advantages but also limitations of each vector system.
Keywords: hybrid vector; somatic integration; adenovirus; retrovirus; adeno-associated virus; herpes simplex virus; targeted integration; Sleeping Beauty transposase; PhiC31 integrase; zinc-finger nuclease
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Müther, N.; Noske, N.; Ehrhardt, A. Viral Hybrid Vectors for Somatic Integration - Are They the Better Solution? Viruses 2009, 1, 1295-1324.
Müther N, Noske N, Ehrhardt A. Viral Hybrid Vectors for Somatic Integration - Are They the Better Solution? Viruses. 2009; 1(3):1295-1324.
Müther, Nadine; Noske, Nadja; Ehrhardt, Anja. 2009. "Viral Hybrid Vectors for Somatic Integration - Are They the Better Solution?" Viruses 1, no. 3: 1295-1324.