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Modified Titanium Surface-Mediated Effects on Human Bone Marrow Stromal Cell Response
BIOMAT Research Cluster, Department of Oral Health Sciences and Prosthetic Dentistry, KU Leuven and University Hospitals Leuven, Kapucijnenvoer 7 box 7001, Leuven 3000, Belgium
Department of Metallurgy and Materials Engineering (MTM), KU Leuven, Kasteelpark Arenberg 44 box 2450, Heverlee 3001, Belgium
Laboratory of Bioengineering and Biomechanics for Bone Articulation, Faculty of Medicine, University Paris Diderot, 10 Avenue de Verdun, Paris 75010, France
Center of Surface Chemistry and Catalysis, KU Leuven, Kasteelpark Arenberg 23 box 2461, Heverlee 3001, Belgium
* Author to whom correspondence should be addressed.
Received: 24 October 2013; in revised form: 7 November 2013 / Accepted: 25 November 2013 / Published: 28 November 2013
Abstract: Surface modification of titanium implants is used to enhance osseointegration. The study objective was to evaluate five modified titanium surfaces in terms of cytocompatibility and pro-osteogenic/pro-angiogenic properties for human mesenchymal stromal cells: amorphous microporous silica (AMS), bone morphogenetic protein-2 immobilized on AMS (AMS + BMP), bio-active glass (BAG) and two titanium coatings with different porosity (T1; T2). Four surfaces served as controls: uncoated Ti (Ti), Ti functionalized with BMP-2 (Ti + BMP), Ti surface with a thickened titanium oxide layer (TiO2) and a tissue culture polystyrene surface (TCPS). The proliferation of eGFP-fLuc (enhanced green fluorescence protein-firefly luciferase) transfected cells was tracked non-invasively by fluorescence microscopy and bio-luminescence imaging. The implant surface-mediated effects on cell differentiation potential was tracked by determination of osteogenic and angiogenic parameters [alkaline phosphatase (ALP); osteocalcin (OC); osteoprotegerin (OPG); vascular endothelial growth factor-A (VEGF-A)]. Unrestrained cell proliferation was observed on (un)functionalized Ti and AMS surfaces, whereas BAG and porous titanium coatings T1 and T2 did not support cell proliferation. An important pro-osteogenic and pro-angiogenic potential of the AMS + BMP surface was observed. In contrast, coating the Ti surface with BMP did not affect the osteogenic differentiation of the progenitor cells. A significantly slower BMP-2 release from AMS compared to Ti supports these findings. In the unfunctionalized state, Ti was found to be superior to AMS in terms of OPG and VEGF-A production. AMS is suggested to be a promising implant coating material for bioactive agents delivery.
Keywords: titanium; surface coating; human bone marrow stromal cells; in vitro cytocompatibility; osteogenic differentiation; osseointegration
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Chaudhari, A.; Duyck, J.; Braem, A.; Vleugels, J.; Petite, H.; Logeart-Avramoglou, D.; Naert, I.; Martens, J.A.; Vandamme, K. Modified Titanium Surface-Mediated Effects on Human Bone Marrow Stromal Cell Response. Materials 2013, 6, 5533-5548.
Chaudhari A, Duyck J, Braem A, Vleugels J, Petite H, Logeart-Avramoglou D, Naert I, Martens JA, Vandamme K. Modified Titanium Surface-Mediated Effects on Human Bone Marrow Stromal Cell Response. Materials. 2013; 6(12):5533-5548.
Chaudhari, Amol; Duyck, Joke; Braem, Annabel; Vleugels, Jozef; Petite, Hervé; Logeart-Avramoglou, Delphine; Naert, Ignace; Martens, Johan A.; Vandamme, Katleen. 2013. "Modified Titanium Surface-Mediated Effects on Human Bone Marrow Stromal Cell Response." Materials 6, no. 12: 5533-5548.