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Materials 2013, 6(12), 5517-5532; doi:10.3390/ma6125517
Article

The Effect of Exogenous Zinc Concentration on the Responsiveness of MC3T3-E1 Pre-Osteoblasts to Surface Microtopography: Part I (Migration)

1
,
1
,
2
,
1,3
 and
1,3,*
1 Department of Materials Science and Engineering, Stony Brook University, Stony Brook, NY 11794-2275, USA 2 Department of Life Sciences, New York Institute of Technology, Old Westbury, NY 11568-8000, USA 3 Department of Chemical and Molecular Engineering, Stony Brook University, Stony Brook, NY 11794-2275, USA
* Author to whom correspondence should be addressed.
Received: 21 October 2013 / Revised: 11 November 2013 / Accepted: 21 November 2013 / Published: 27 November 2013
(This article belongs to the Special Issue Biocompatibility of Materials 2013)
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Abstract

Initial cell-surface interactions are guided by the material properties of substrate topography. To examine if these interactions are also modulated by the presence of zinc, we seeded murine pre-osteoblasts (MC3T3-E1, subclone 4) on micropatterned polydimethylsiloxane (PDMS) containing wide (20 µm width, 30 µm pitch, 2 µm height) or narrow (2 µm width, 10 µm pitch, 2 µm height) ridges, with flat PDMS and tissue culture polystyrene (TC) as controls. Zinc concentration was adjusted to mimic deficient (0.23 µM), serum-level (3.6 µM), and zinc-rich (50 µM) conditions. Significant differences were observed in regard to cell morphology, motility, and contact guidance. We found that cells exhibited distinct anisotropic migration on the wide PDMS patterns under either zinc-deprived (0.23 µM) or serum-level zinc conditions (3.6 µM). However, this effect was absent in a zinc-rich environment (50 µM). These results suggest that the contact guidance of pre-osteoblasts may be partly influenced by trace metals in the microenvironment of the extracellular matrix.
Keywords: zinc; osteoblast; polydimethylsiloxane; micropatterns; contact guidance zinc; osteoblast; polydimethylsiloxane; micropatterns; contact guidance
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Dorst, K.; Rammelkamp, D.; Hadjiargyrou, M.; Gersappe, D.; Meng, Y. The Effect of Exogenous Zinc Concentration on the Responsiveness of MC3T3-E1 Pre-Osteoblasts to Surface Microtopography: Part I (Migration). Materials 2013, 6, 5517-5532.

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