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Materials 2013, 6(11), 5398-5409; doi:10.3390/ma6115398
Article

Comparative Analysis of the Oxygen Supply and Viability of Human Osteoblasts in Three-Dimensional Titanium Scaffolds Produced by Laser-Beam or Electron-Beam Melting

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Received: 8 October 2013; in revised form: 1 November 2013 / Accepted: 14 November 2013 / Published: 21 November 2013
(This article belongs to the Special Issue Titanium Materials for Biomedical Application 2013)
Download PDF [391 KB, uploaded 21 November 2013]
Abstract: Synthetic materials for bone replacement must ensure a sufficient mechanical stability and an adequate cell proliferation within the structures. Hereby, titanium materials are suitable for producing patient-individual porous bone scaffolds by using generative techniques. In this in vitro study, the viability of human osteoblasts was investigated in porous 3D Ti6Al4V scaffolds, which were produced by electron-beam (EBM) or laser-beam melting (LBM). For each examination, two cylindrical scaffolds (30 mm × 10 mm in size, 700 µm × 700 µm macropores) were placed on each other and seeded with cells. The oxygen consumption and the acidification in the center of the structures were investigated by means of microsensors. Additionally, the synthesis of pro-collagen type 1 was analyzed. On the LBM titanium scaffolds, vital bone cells were detected in the center and in the periphery after 8 days of cultivation. In the EBM titanium constructs, however, vital cells were only visible in the center. During the cultivation period, the cells increasingly produced procollagen type 1 in both scaffolds. In comparison to the periphery, the oxygen content in the center of the scaffolds slightly decreased. Furthermore, a slight acidification of the medium was detectable. Compared to LBM, the EBM titanium scaffolds showed a less favorable behavior with regard to cell seeding.
Keywords: human osteoblasts; Ti6Al4V; laser-beam melting; electron-beam melting; scaffold; macropores human osteoblasts; Ti6Al4V; laser-beam melting; electron-beam melting; scaffold; macropores
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Jonitz-Heincke, A.; Wieding, J.; Schulze, C.; Hansmann, D.; Bader, R. Comparative Analysis of the Oxygen Supply and Viability of Human Osteoblasts in Three-Dimensional Titanium Scaffolds Produced by Laser-Beam or Electron-Beam Melting. Materials 2013, 6, 5398-5409.

AMA Style

Jonitz-Heincke A, Wieding J, Schulze C, Hansmann D, Bader R. Comparative Analysis of the Oxygen Supply and Viability of Human Osteoblasts in Three-Dimensional Titanium Scaffolds Produced by Laser-Beam or Electron-Beam Melting. Materials. 2013; 6(11):5398-5409.

Chicago/Turabian Style

Jonitz-Heincke, Anika; Wieding, Jan; Schulze, Christoph; Hansmann, Doris; Bader, Rainer. 2013. "Comparative Analysis of the Oxygen Supply and Viability of Human Osteoblasts in Three-Dimensional Titanium Scaffolds Produced by Laser-Beam or Electron-Beam Melting." Materials 6, no. 11: 5398-5409.


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