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Molecular Epidemiology for Vector Research on Leishmaniasis
Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan
Departamento de Oncocercosis, Servicio Nacional de Erradicacion de la Malaria, Ministerio de Salud Publica, Guayaquil 10833, Ecuador
Sección de Entomología, Instituto de Medicina Tropical “Daniel A. Carrion”, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Lima 1, Perú
Laboratorio de Entomología, Instituto Nacional de Salud, Lima, Lima 11, Perú
Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa 903-0125, Japan
Department of Microbiology, School of Health Sciences, Kumamoto University, Kumamoto 862-0976, Japan
Department of Parasitology, Kochi Medical School, Kochi University, Kochi 783-8505, Japan
* Author to whom correspondence should be addressed.
Received: 9 December 2009; in revised form: 3 February 2010 / Accepted: 16 February 2010 / Published: 5 March 2010
Abstract: Leishmaniasis is a protozoan disease caused by the genus Leishmania transmitted by female phlebotomine sand flies. Surveillance of the prevalence of Leishmania and responsive vector species in endemic and surrounding areas is important for predicting the risk and expansion of the disease. Molecular biological methods are now widely applied to epidemiological studies of infectious diseases including leishmaniasis. These techniques are used to detect natural infections of sand fly vectors with Leishmania protozoa and are becoming powerful tools due to their sensitivity and specificity. Recently, genetic analyses have been performed on sand fly species and genotyping using PCR-RFLP has been applied to the sand fly taxonomy. In addition, a molecular mass screening method has been established that enables both sand fly species and natural leishmanial infections to be identified simultaneously in hundreds of sand flies with limited effort. This paper reviews recent advances in the study of sand flies, vectors of leishmaniasis, using molecular biological approaches.
Keywords: Leishmania; sand fly; epidemiology; mass screening
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Kato, H.; Gomez, E.A.; Cáceres, A.G.; Uezato, H.; Mimori, T.; Hashiguchi, Y. Molecular Epidemiology for Vector Research on Leishmaniasis. Int. J. Environ. Res. Public Health 2010, 7, 814-826.
Kato H, Gomez EA, Cáceres AG, Uezato H, Mimori T, Hashiguchi Y. Molecular Epidemiology for Vector Research on Leishmaniasis. International Journal of Environmental Research and Public Health. 2010; 7(3):814-826.
Kato, Hirotomo; Gomez, Eduardo A.; Cáceres, Abraham G.; Uezato, Hiroshi; Mimori, Tatsuyuki; Hashiguchi, Yoshihisa. 2010. "Molecular Epidemiology for Vector Research on Leishmaniasis." Int. J. Environ. Res. Public Health 7, no. 3: 814-826.