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Int. J. Environ. Res. Public Health 2016, 13(5), 461; doi:10.3390/ijerph13050461

Use of Walnut Shell Powder to Inhibit Expression of Fe2+-Oxidizing Genes of Acidithiobacillus Ferrooxidans

1
Department of Biological and Environmental Engineering, Hefei University, Hefei 230601, China
2
School of Public Health, Anhui Medical University, Hefei 230032, China
*
Author to whom correspondence should be addressed.
Academic Editors: Nicholas Frederick Gray and Panagiotis Karanis
Received: 14 February 2016 / Revised: 12 April 2016 / Accepted: 15 April 2016 / Published: 30 April 2016
(This article belongs to the Special Issue Water Microbial Pollution and Disinfection)
View Full-Text   |   Download PDF [4508 KB, uploaded 30 April 2016]   |  

Abstract

Acidithiobacillus ferrooxidans is a Gram-negative bacterium that obtains energy by oxidizing Fe2+ or reduced sulfur compounds. This bacterium contributes to the formation of acid mine drainage (AMD). This study determined whether walnut shell powder inhibits the growth of A. ferrooxidans. First, the effects of walnut shell powder on Fe2+ oxidization and H+ production were evaluated. Second, the chemical constituents of walnut shell were isolated to determine the active ingredient(s). Third, the expression of Fe2+-oxidizing genes and rus operon genes was investigated using real-time polymerase chain reaction. Finally, growth curves were plotted, and a bioleaching experiment was performed to confirm the active ingredient(s) in walnut shells. The results indicated that both walnut shell powder and the phenolic fraction exert high inhibitory effects on Fe2+ oxidation and H+ production by A. ferrooxidans cultured in standard 9K medium. The phenolic components exert their inhibitory effects by down-regulating the expression of Fe2+-oxidizing genes and rus operon genes, which significantly decreased the growth of A. ferrooxidans. This study revealed walnut shell powder to be a promising substance for controlling AMD. View Full-Text
Keywords: walnut shell powder; acid mine drainage; Acidithiobacillus ferrooxidans; rus operon walnut shell powder; acid mine drainage; Acidithiobacillus ferrooxidans; rus operon
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, Y.; Liu, Y.; Tan, H.; Zhang, Y.; Yue, M. Use of Walnut Shell Powder to Inhibit Expression of Fe2+-Oxidizing Genes of Acidithiobacillus Ferrooxidans. Int. J. Environ. Res. Public Health 2016, 13, 461.

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