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Int. J. Environ. Res. Public Health 2016, 13(4), 426; doi:10.3390/ijerph13040426

Isolation and Characterization of Aquatic-Borne Klebsiella pneumoniae from Tropical Estuaries in Malaysia

1
Faculty of Science, Institute of Biological Sciences, University of Malaya, Kuala Lumpur 50603, Malaysia
2
Department of Biomedical Sciences, Wide River Institute of Immunology, Seoul National University College of Medicine, Seoul 03080, Korea
3
Institute of Ocean & Earth Sciences, University of Malaya, Kuala Lumpur 50603, Malaysia
4
Faculty of Applied Sciences, UCSI University, Kuala Lumpur 56000, Malaysia
*
Author to whom correspondence should be addressed.
Academic Editors: Nicholas Frederick Gray and Panagiotis Karanis
Received: 26 July 2015 / Revised: 23 March 2016 / Accepted: 31 March 2016 / Published: 15 April 2016
(This article belongs to the Special Issue Water Microbial Pollution and Disinfection)
View Full-Text   |   Download PDF [1398 KB, uploaded 15 April 2016]   |  

Abstract

Klebsiella pneumoniae is an opportunistic pathogen that is responsible for causing nosocomial and community-acquired infections. Despite its common presence in soil and aquatic environments, the virulence potential of K. pneumoniae isolates of environmental origin is largely unknown. Hence, in this study, K. pneumoniae isolated from the estuarine waters and sediments of the Matang mangrove estuary were screened for potential virulence characteristics: antibiotic susceptibility, morphotype on Congo red agar, biofilm formation, presence of exopolysaccharide and capsule, possession of virulence genes (fimH, magA, ugE, wabG and rmpA) and their genomic fingerprints. A total of 55 strains of K. pneumoniae were isolated from both human-distributed sites (located along Sangga Besar River) and control sites (located along Selinsing River) where less human activity was observed, indicated that K. pneumoniae is ubiquitous in the environment. However, the detection of potentially virulent strains at the downstream of Kuala Sepetang village has suggested an anthropogenic contamination source. In conclusion, the findings from this study indicate that the Matang mangrove estuary could harbor potentially pathogenic K. pneumoniae with risk to public health. More studies are required to compare the environmental K. pneumoniae strains with the community-acquired K. pneumoniae strains. View Full-Text
Keywords: Klebsiella pneumoniae; aquatic; anthropogenic contamination; antimicrobial resistance; virulence factors Klebsiella pneumoniae; aquatic; anthropogenic contamination; antimicrobial resistance; virulence factors
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Barati, A.; Ghaderpour, A.; Chew, L.L.; Bong, C.W.; Thong, K.L.; Chong, V.C.; Chai, L.C. Isolation and Characterization of Aquatic-Borne Klebsiella pneumoniae from Tropical Estuaries in Malaysia. Int. J. Environ. Res. Public Health 2016, 13, 426.

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