Supraphysiological Levels of Quercetin Glycosides are Required to Alter Mineralization in Saos2 Cells
AbstractFlavonoid intake is positively correlated to bone mineral density (BMD) in women. Flavonoids such as quercetin exhibit strong anti-oxidant and anti-inflammatory activity that may be beneficial for bone health. Quercetin, previously shown to positively influence osteoblasts, is metabolized into glycosides including rutin and hyperoside. We compared the effects of these glycosides on mineralization in human osteoblast (Saos2) cells. Administration of rutin (≥25 µM) and hyperoside (≥5 µM) resulted in higher mineral content, determined using the alizarin red assay. This was accompanied by higher alkaline phosphatase activity with no cell toxicity. The expression of osteopontin, sclerostin, TNFα and IL6, known stimuli for decreasing osteoblast activity, were reduced with the addition of rutin or hyperoside. In summary, rutin and hyperoside require supraphysiological levels, when administered individually, to positively influence osteoblast activity. This information may be useful in developing nutraceuticals to support bone health. View Full-Text
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Nash, L.A.; Peters, S.J.; Sullivan, P.J.; Ward, W.E. Supraphysiological Levels of Quercetin Glycosides are Required to Alter Mineralization in Saos2 Cells. Int. J. Environ. Res. Public Health 2016, 13, 460.
Nash LA, Peters SJ, Sullivan PJ, Ward WE. Supraphysiological Levels of Quercetin Glycosides are Required to Alter Mineralization in Saos2 Cells. International Journal of Environmental Research and Public Health. 2016; 13(5):460.Chicago/Turabian Style
Nash, Leslie A.; Peters, Sandra J.; Sullivan, Philip J.; Ward, Wendy E. 2016. "Supraphysiological Levels of Quercetin Glycosides are Required to Alter Mineralization in Saos2 Cells." Int. J. Environ. Res. Public Health 13, no. 5: 460.
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