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Int. J. Environ. Res. Public Health 2014, 11(8), 7953-7960; doi:10.3390/ijerph110807953

Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts

Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies
International Research Institute for Climate and Society, The Earth Institute, Columbia University, Palisades, NY 10964, USA
Current address: Department of Microbiology & Immunology, Midwestern University, 19555 North 59th Avenue, Glendale, AZ 85308, USA
Author to whom correspondence should be addressed.
Received: 31 May 2014 / Revised: 26 June 2014 / Accepted: 1 July 2014 / Published: 7 August 2014
(This article belongs to the Special Issue Leptospirosis in the Animal—Human-Ecosystem Interface)
View Full-Text   |   Download PDF [338 KB, uploaded 7 August 2014]   |  


Leptospirosis is an important waterborne zoonotic disease caused by pathogenic Leptospira. The pathogen is maintained in a population due to chronic colonization and shedding from renal tubules of domestic and wild animals. Humans and other animals become infected when they come in contact with urine from infected animals, either directly or through urine-contaminated surface water. In this study, we screened environmental water on the island of St. Kitts by using a TaqMan based real time quantitative polymerase chain reaction (qPCR) targeting a pathogen specific leptospiral gene, lipl32. Our results indicate that around one-fifth of tested water sources have detectable leptospiral DNA. View Full-Text
Keywords: Leptospira; molecular detection; environmental transmission; leptospirosis Leptospira; molecular detection; environmental transmission; leptospirosis

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MDPI and ACS Style

Rawlins, J.; Portanova, A.; Zuckerman, I.; Loftis, A.; Ceccato, P.; Willingham, A.L.; Verma, A. Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts. Int. J. Environ. Res. Public Health 2014, 11, 7953-7960.

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