Next Article in Journal
Effectiveness of a New Exercise Program after Lower Limb Arterial Blood Flow Surgery in Patients with Peripheral Arterial Disease: A Randomized Clinical Trial
Next Article in Special Issue
Socioeconomic Factors and Vulnerability to Outbreaks of Leptospirosis in Nicaragua
Previous Article in Journal
Reproductive Outcomes Associated with Noise Exposure — A Systematic Review of the Literature
Previous Article in Special Issue
Leptospira spp. in Rodents and Shrews in Germany
Article Menu

Export Article

Open AccessCommunication
Int. J. Environ. Res. Public Health 2014, 11(8), 7953-7960; doi:10.3390/ijerph110807953

Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts

1
Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies
2
International Research Institute for Climate and Society, The Earth Institute, Columbia University, Palisades, NY 10964, USA
Current address: Department of Microbiology & Immunology, Midwestern University, 19555 North 59th Avenue, Glendale, AZ 85308, USA
*
Author to whom correspondence should be addressed.
Received: 31 May 2014 / Revised: 26 June 2014 / Accepted: 1 July 2014 / Published: 7 August 2014
(This article belongs to the Special Issue Leptospirosis in the Animal—Human-Ecosystem Interface)
View Full-Text   |   Download PDF [338 KB, uploaded 7 August 2014]   |  

Abstract

Leptospirosis is an important waterborne zoonotic disease caused by pathogenic Leptospira. The pathogen is maintained in a population due to chronic colonization and shedding from renal tubules of domestic and wild animals. Humans and other animals become infected when they come in contact with urine from infected animals, either directly or through urine-contaminated surface water. In this study, we screened environmental water on the island of St. Kitts by using a TaqMan based real time quantitative polymerase chain reaction (qPCR) targeting a pathogen specific leptospiral gene, lipl32. Our results indicate that around one-fifth of tested water sources have detectable leptospiral DNA. View Full-Text
Keywords: Leptospira; molecular detection; environmental transmission; leptospirosis Leptospira; molecular detection; environmental transmission; leptospirosis
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Rawlins, J.; Portanova, A.; Zuckerman, I.; Loftis, A.; Ceccato, P.; Willingham, A.L.; Verma, A. Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts. Int. J. Environ. Res. Public Health 2014, 11, 7953-7960.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top