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Int. J. Environ. Res. Public Health 2013, 10(7), 2688-2698; doi:10.3390/ijerph10072688

Detection of β-Lactamase Residues in Milk by Sandwich ELISA

*  and
State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China
* Author to whom correspondence should be addressed.
Received: 16 May 2013 / Revised: 9 June 2013 / Accepted: 17 June 2013 / Published: 28 June 2013
(This article belongs to the Special Issue Food Safety and Public Health)
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β-Lactamase residues in milk represent a public health risk. The cylinder plate detection method, which is based on bacterial growth, is laborious and time consuming. In this study, 15 monoclonal antibodies (mAbs) were selected against Temoneira (TEM) 1 β-lactamase. A sandwich enzyme-linked immunosorbent assay (ELISA) based on an optimum mAb pair was developed and validated for the detection of β-lactamase. The limit of detection and linear dynamic range of the method were 4.17 ng/mL and 5.5–100 ng/mL, respectively. β-Lactamase recovery in pure milk was 96.82–103.13%. The intra- and inter-assay coefficients of variation were 6.21–7.38% and 12.96–13.74%, respectively. Our developed sandwich ELISA can be used as a rapid detection method of β-lactamase in milk.
Keywords: β-lactamase; monoclonal antibody; sandwich ELISA; milk; detection β-lactamase; monoclonal antibody; sandwich ELISA; milk; detection
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Wang, W.; Liu, L.; Xu, L.; Ma, W.; Kuang, H.; Xu, C. Detection of β-Lactamase Residues in Milk by Sandwich ELISA. Int. J. Environ. Res. Public Health 2013, 10, 2688-2698.

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