Mar. Drugs 2011, 9(5), 889-905; doi:10.3390/md9050889
Article

Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey

Received: 20 April 2011; in revised form: 17 May 2011 / Accepted: 20 May 2011 / Published: 24 May 2011
(This article belongs to the Special Issue Enzymes from the Sea: Sources, Molecular Biology and Bioprocesses)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (RS)-1-(phenyl)ethanol as substrates. Among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze the (S)-enantiomer oxidation from racemic alcohols to their corresponding ketones. Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity. These new bacteria strains were selected for optimization study, in which the (RS)-1-(4-methyl-phenyl)ethanol oxidation was evaluated in several reaction conditions. From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C. We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph.
Keywords: oxidoreductases; chiral alcohols; psychrophile; psychrotroph; oxidation; deracemisation
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MDPI and ACS Style

Araújo, L.S.; Kagohara, E.; Garcia, T.P.; Pellizari, V.H.; Andrade, L.H. Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey. Mar. Drugs 2011, 9, 889-905.

AMA Style

Araújo LS, Kagohara E, Garcia TP, Pellizari VH, Andrade LH. Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey. Marine Drugs. 2011; 9(5):889-905.

Chicago/Turabian Style

Araújo, Lidiane S.; Kagohara, Edna; Garcia, Thaís P.; Pellizari, Vivian H.; Andrade, Leandro H. 2011. "Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey." Mar. Drugs 9, no. 5: 889-905.

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