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Mar. Drugs 2011, 9(1), 82-97; doi:10.3390/md9010082

Search for Hydrophilic Marine Fungal Metabolites: A Rational Approach for Their Production and Extraction in a Bioactivity Screening Context

University of Nantes, Faculty of Pharmacy, MMS–EA 2160, F-44000 Nantes, France
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Received: 18 December 2010 / Revised: 30 December 2010 / Accepted: 5 January 2011 / Published: 10 January 2011
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Abstract

In the search for bioactive natural products, our lab screens hydrophobic extracts from marine fungal strains. While hydrophilic active substances were recently identified from marine macro-organisms, there was a lack of reported metabolites in the marine fungi area. As such, we decided to develop a general procedure for screening of hydrophobic metabolites. The aim of this study was to compare different processes of fermentation and extraction, using six representative marine fungal strains, in order to define the optimized method for production. The parameters studied were (a) which polar solvent to select, (b) which fermentation method to choose between solid and liquid cultures, (c) which raw material, the mycelium or its medium, to extract and (d) which extraction process to apply. The biochemical analysis and biological evaluations of obtained extracts led to the conclusion that the culture of marine fungi by agar surface fermentation followed by the separate extraction of the mycelium and its medium by a cryo-crushing and an enzymatic digestion with agarase, respectively, was the best procedure when screening for hydrophilic bioactive metabolites. During this development, several bioactivities were detected, confirming the potential of hydrophilic crude extracts in the search for bioactive natural products. View Full-Text
Keywords: marine fungi; hydrophilic metabolites; bioactivity; agar surface fermentation; submerged fermentation; extraction process marine fungi; hydrophilic metabolites; bioactivity; agar surface fermentation; submerged fermentation; extraction process
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Ker, C.L.; Petit, K.-E.; Biard, J.-F.; Fleurence, J. Search for Hydrophilic Marine Fungal Metabolites: A Rational Approach for Their Production and Extraction in a Bioactivity Screening Context. Mar. Drugs 2011, 9, 82-97.

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