Abstract: The starfish Narcissia canariensis harvested from the coasts off Dakar, Senegal, was investigated for glycolipids (GL). This report deals with the isolation, characterization and biological activity of a fraction F13-3 separated from the GL mixture and selected according to its ability to inhibit KB cell proliferation after 72 hours of treatment. Firstly, a GL mixture F13 was obtained that accounted for 1.36% of starfish biomass (dry weight) and 0.36% of total lipids. The fraction F13-3 obtained from F13 contained three homologous GL identified as peracetylated derivatives on the basis of chemical and spectroscopic evidence. These contained a β-glucopyranoside as sugar head, a 9-methyl-branched 4,8,10-triunsaturated long-chain aminoalcohol as sphingoid base and amide-linked 2-hydroxy fatty acid chains. The majority (63%) had an amide-linked 2‑hydroxydocosanoic acid chain and was identified as the ophidiacerebroside-C, firstly isolated from the starfish Ophidiaster ophidiamus. The minor components of F13-3 differed by one more or one less methylene group, and corresponded to ophidiacerebroside-B and -D. We found that F13-3 displayed an interesting cytotoxic activity over 24 hours on various adherent human cancerous cell lines (multiple myeloma, colorectal adenocarcinoma and glioblastoma multiforme) with an IC50 of around 20 μM.
Keywords: glycosylceramides; human cancer cell lines; in vitro anticancerous activity; Narcissia canariensis; starfish
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Farokhi, F.; Wielgosz-Collin, G.; Clement, M.; Kornprobst, J.-M.; Barnathan, G. Cytotoxicity on Human Cancer Cells of Ophidiacerebrosides Isolated from the African Starfish Narcissia canariensis. Mar. Drugs 2010, 8, 2988-2998.
Farokhi F, Wielgosz-Collin G, Clement M, Kornprobst J-M, Barnathan G. Cytotoxicity on Human Cancer Cells of Ophidiacerebrosides Isolated from the African Starfish Narcissia canariensis. Marine Drugs. 2010; 8(12):2988-2998.
Farokhi, Fereshteh; Wielgosz-Collin, Gaetane; Clement, Monique; Kornprobst, Jean-Michel; Barnathan, Gilles. 2010. "Cytotoxicity on Human Cancer Cells of Ophidiacerebrosides Isolated from the African Starfish Narcissia canariensis." Mar. Drugs 8, no. 12: 2988-2998.