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Mar. Drugs 2014, 12(5), 2790-2801; doi:10.3390/md12052790
Article

Marine Compound Catunaregin Inhibits Angiogenesis through the Modulation of Phosphorylation of Akt and eNOS in vivo and in vitro

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Received: 10 February 2014; in revised form: 9 April 2014 / Accepted: 14 April 2014 / Published: 12 May 2014
(This article belongs to the Special Issue Advances and New Perspectives in Marine Biotechnology)
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Abstract: Angiogenesis is the formation of blood vessels from pre-existing vasculature. Excessive or uncontrolled angiogenesis is a major contributor to many pathological conditions whereas inhibition of aberrant angiogenesis is beneficial to patients with pathological angiogenesis. Catunaregin is a core of novel marine compound isolated from mangrove associate. The potential anti-angiogenesis of catunaregin was investigated in human umbilical vein endothelial cells (HUVECs) and zebrafish. HUVECs were treated with different concentrations of catunaregin in the presence or absence of VEGF. The angiogenic phenotypes including cell invasion cell migration and tube formation were evaluated following catunaregin treatment in HUVECs. The possible involvement of AKT, eNOS and ERK1/2 in catunaregin-induced anti-angiogenesis was explored using Western blotting. The anti-angiogenesis of catunaregin was further tested in the zebrafish embryo neovascularization and caudal fin regeneration assays. We found that catunaregin dose-dependently inhibited angiogenesis in both HUVECs and zebrafish embryo neovascularization and zebrafish caudal fin regeneration assays. In addition, catunaregin significantly decreased the phosphorylation of Akt and eNOS, but not the phosphorylation of ERK1/2. The present work demonstrates that catunaregin exerts the anti-angiogenic activity at least in part through the regulation of the Akt and eNOS signaling pathways.
Keywords: anti-angiogenesis; catunaregin; VEGF; zebrafish; HUVECs anti-angiogenesis; catunaregin; VEGF; zebrafish; HUVECs
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Liu, J.-X.; Luo, M.-Q.; Xia, M.; Wu, Q.; Long, S.-M.; Hu, Y.; Gao, G.-C.; Yao, X.-L.; He, M.; Su, H.; Luo, X.-M.; Yao, S.-Z. Marine Compound Catunaregin Inhibits Angiogenesis through the Modulation of Phosphorylation of Akt and eNOS in vivo and in vitro. Mar. Drugs 2014, 12, 2790-2801.

AMA Style

Liu J-X, Luo M-Q, Xia M, Wu Q, Long S-M, Hu Y, Gao G-C, Yao X-L, He M, Su H, Luo X-M, Yao S-Z. Marine Compound Catunaregin Inhibits Angiogenesis through the Modulation of Phosphorylation of Akt and eNOS in vivo and in vitro. Marine Drugs. 2014; 12(5):2790-2801.

Chicago/Turabian Style

Liu, Jun-Xiu; Luo, Min-Qi; Xia, Meng; Wu, Qi; Long, Si-Mei; Hu, Yaohua; Gao, Guang-Chun; Yao, Xiao-Li; He, Mian; Su, Huanxing; Luo, Xiong-Ming; Yao, Shu-Zhong. 2014. "Marine Compound Catunaregin Inhibits Angiogenesis through the Modulation of Phosphorylation of Akt and eNOS in vivo and in vitro." Mar. Drugs 12, no. 5: 2790-2801.


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