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Mar. Drugs 2014, 12(3), 1530-1544; doi:10.3390/md12031530
Article

CS5931, a Novel Polypeptide in Ciona savignyi, Represses Angiogenesis via Inhibiting Vascular Endothelial Growth Factor (VEGF) and Matrix Metalloproteinases (MMPs)

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Received: 15 November 2013; in revised form: 9 January 2014 / Accepted: 22 January 2014 / Published: 13 March 2014
(This article belongs to the Special Issue Advances and New Perspectives in Marine Biotechnology)
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Abstract: CS5931 is a novel polypeptide from Ciona savignyi with anticancer activities. Previous study in our laboratory has shown that CS5931 can induce cell death via mitochondrial apoptotic pathway. In the present study, we found that the polypeptide could inhibit angiogenesis both in vitro and in vivo. CS5931 inhibited the proliferation, migration and formation of capillary-like structures of HUVECs (Human Umbilical Vein Endothelial Cell) in a dose-dependent manner. Additionally, CS5931 repressed spontaneous angiogenesis of the zebrafish vessels. Further studies showed that CS5931 also blocked vascular endothelial growth factor (VEGF) production but without any effect on its mRNA expression. Moreover, CS5931 reduced the expression of matrix metalloproteinases (MMP-2 and MMP-9) both on protein and mRNA levels in HUVEC cells. We demonstrated that CS5931 possessed strong anti-angiogenic activity both in vitro and in vivo, possible via VEGF and MMPs. This study indicates that CS5931 has the potential to be developed as a novel therapeutic agent as an inhibitor of angiogenesis for the treatment of cancer.
Keywords: CS5931; anti-angiogenesis; zebrafish; HUVECs; VEGF and MMPs CS5931; anti-angiogenesis; zebrafish; HUVECs; VEGF and MMPs
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Liu, G.; Liu, M.; Wei, J.; Huang, H.; Zhang, Y.; Zhao, J.; Xiao, L.; Wu, N.; Zheng, L.; Lin, X. CS5931, a Novel Polypeptide in Ciona savignyi, Represses Angiogenesis via Inhibiting Vascular Endothelial Growth Factor (VEGF) and Matrix Metalloproteinases (MMPs). Mar. Drugs 2014, 12, 1530-1544.

AMA Style

Liu G, Liu M, Wei J, Huang H, Zhang Y, Zhao J, Xiao L, Wu N, Zheng L, Lin X. CS5931, a Novel Polypeptide in Ciona savignyi, Represses Angiogenesis via Inhibiting Vascular Endothelial Growth Factor (VEGF) and Matrix Metalloproteinases (MMPs). Marine Drugs. 2014; 12(3):1530-1544.

Chicago/Turabian Style

Liu, Ge; Liu, Ming; Wei, Jianteng; Huang, Haijuan; Zhang, Yuyan; Zhao, Jin; Xiao, Lin; Wu, Ning; Zheng, Lanhong; Lin, Xiukun. 2014. "CS5931, a Novel Polypeptide in Ciona savignyi, Represses Angiogenesis via Inhibiting Vascular Endothelial Growth Factor (VEGF) and Matrix Metalloproteinases (MMPs)." Mar. Drugs 12, no. 3: 1530-1544.



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