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Mar. Drugs 2014, 12(4), 1891-1910; doi:10.3390/md12041891
Article

Combined Effects of Nitrogen Concentration and Seasonal Changes on the Production of Lipids in Nannochloropsis oculata

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Received: 16 January 2014; in revised form: 26 February 2014 / Accepted: 10 March 2014 / Published: 31 March 2014
(This article belongs to the Special Issue Advances and New Perspectives in Marine Biotechnology)
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Abstract: Instead of sole nutrient starvation to boost algal lipid production, we addressed nutrient limitation at two different seasons (autumn and spring) during outdoor cultivation in flat panel photobioreactors. Lipid accumulation, biomass and lipid productivity and changes in fatty acid composition of Nannochloropsis oculata were investigated under nitrogen (N) limitation (nitrate:phosphate N:P 5, N:P 2.5 molar ratio). N. oculata was able to maintain a high biomass productivity under N-limitation compared to N-sufficiency (N:P 20) at both seasons, which in spring resulted in nearly double lipid productivity under N-limited conditions (0.21 g L−1 day−1) compared to N-sufficiency (0.11 g L−1 day−1). Saturated and monounsaturated fatty acids increased from 76% to nearly 90% of total fatty acids in N-limited cultures. Higher biomass and lipid productivity in spring could, partly, be explained by higher irradiance, partly by greater harvesting rate (~30%). Our results indicate the potential for the production of algal high value products (i.e., polyunsaturated fatty acids) during both N-sufficiency and N-limitation. To meet the sustainability challenges of algal biomass production, we propose a dual-system process: Closed photobioreactors producing biomass for high value products and inoculum for larger raceway ponds recycling waste/exhaust streams to produce bulk chemicals for fuel, feed and industrial material.
Keywords: microalgae; outdoor; Nannochloropsis; large-scale; lipids; fatty acids; nitrogen manipulation; seasonal changes; biofuels; high value products microalgae; outdoor; Nannochloropsis; large-scale; lipids; fatty acids; nitrogen manipulation; seasonal changes; biofuels; high value products
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Olofsson, M.; Lamela, T.; Nilsson, E.; Bergé, J.-P.; del Pino, V.; Uronen, P.; Legrand, C. Combined Effects of Nitrogen Concentration and Seasonal Changes on the Production of Lipids in Nannochloropsis oculata. Mar. Drugs 2014, 12, 1891-1910.

AMA Style

Olofsson M, Lamela T, Nilsson E, Bergé J-P, del Pino V, Uronen P, Legrand C. Combined Effects of Nitrogen Concentration and Seasonal Changes on the Production of Lipids in Nannochloropsis oculata. Marine Drugs. 2014; 12(4):1891-1910.

Chicago/Turabian Style

Olofsson, Martin; Lamela, Teresa; Nilsson, Emmelie; Bergé, Jean-Pascal; del Pino, Victória; Uronen, Pauliina; Legrand, Catherine. 2014. "Combined Effects of Nitrogen Concentration and Seasonal Changes on the Production of Lipids in Nannochloropsis oculata." Mar. Drugs 12, no. 4: 1891-1910.


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