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Assessing the Effectiveness of Functional Genetic Screens for the Identification of Bioactive Metabolites
School of Biotechnology and Biomolecular Sciences and Centre for Marine Bio-Innovation, University of New South Wales, Sydney 2052, New South Wales, Australia
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney 2109, New South Wales, Australia
The Singapore Center on Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 18 October 2012; in revised form: 13 November 2012 / Accepted: 12 December 2012 / Published: 27 December 2012
Abstract: A common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. Here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance the screening efficiency, by increasing the “hit-rate” and unmasking multiple activities from the same bacterial source.
Keywords: antibacterial; antinematode; functional genomic screen; fosmid libraries; marine bacteria; marine bioactives; Caenorhabditis elegans
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Penesyan, A.; Ballestriero, F.; Daim, M.; Kjelleberg, S.; Thomas, T.; Egan, S. Assessing the Effectiveness of Functional Genetic Screens for the Identification of Bioactive Metabolites. Mar. Drugs 2013, 11, 40-49.
Penesyan A, Ballestriero F, Daim M, Kjelleberg S, Thomas T, Egan S. Assessing the Effectiveness of Functional Genetic Screens for the Identification of Bioactive Metabolites. Marine Drugs. 2013; 11(1):40-49.
Penesyan, Anahit; Ballestriero, Francesco; Daim, Malak; Kjelleberg, Staffan; Thomas, Torsten; Egan, Suhelen. 2013. "Assessing the Effectiveness of Functional Genetic Screens for the Identification of Bioactive Metabolites." Mar. Drugs 11, no. 1: 40-49.