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Mar. Drugs 2013, 11(1), 40-49; doi:10.3390/md11010040
Article

Assessing the Effectiveness of Functional Genetic Screens for the Identification of Bioactive Metabolites

1,2,†
,
1,†
,
1
,
1,3
,
1
 and
1,*
1 School of Biotechnology and Biomolecular Sciences and Centre for Marine Bio-Innovation, University of New South Wales, Sydney 2052, New South Wales, Australia 2 Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney 2109, New South Wales, Australia 3 The Singapore Center on Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 18 October 2012 / Revised: 13 November 2012 / Accepted: 12 December 2012 / Published: 27 December 2012
(This article belongs to the Special Issue Marine Antibiotics)
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Abstract

A common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. Here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance the screening efficiency, by increasing the “hit-rate” and unmasking multiple activities from the same bacterial source.
Keywords: antibacterial; antinematode; functional genomic screen; fosmid libraries; marine bacteria; marine bioactives; Caenorhabditis elegans antibacterial; antinematode; functional genomic screen; fosmid libraries; marine bacteria; marine bioactives; Caenorhabditis elegans
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Penesyan, A.; Ballestriero, F.; Daim, M.; Kjelleberg, S.; Thomas, T.; Egan, S. Assessing the Effectiveness of Functional Genetic Screens for the Identification of Bioactive Metabolites. Mar. Drugs 2013, 11, 40-49.

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