Mar. Drugs 2013, 11(1), 50-66; doi:10.3390/md11010050
Article

Fucoxanthin Enhances Cisplatin-Induced Cytotoxicity via NFκB-Mediated Pathway and Downregulates DNA Repair Gene Expression in Human Hepatoma HepG2 Cells

1 Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 402, Taiwan 2 Department of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan 3 Department of Emergency, Toxicology Center, China Medical University Hospital, Taichung 404, Taiwan 4 Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan
* Author to whom correspondence should be addressed.
Received: 10 October 2012; in revised form: 14 November 2012 / Accepted: 13 December 2012 / Published: 8 January 2013
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Abstract: Cisplain, a platinum-containing anticancer drug, has been shown to enhance DNA repair and to inhibit cell apoptosis, leading to drug resistance. Thus, the combination of anticancer drugs with nutritional factors is a potential strategy for improving the efficacy of cisplatin chemotherapy. In this study, we investigated the anti-proliferative effects of a combination of fucoxanthin, the major non-provitamin A carotenoid found in Undaria Pinnatifida, and cisplatin in human hepatoma HepG2 cells. We found that fucoxanthin (1–10 μΜ) pretreatment for 24 h followed by cisplatin (10 μΜ) for 24 h significantly decreased cell proliferation, as compared with cisplatin treatment alone. Mechanistically, we showed that fucoxanthin attenuated cisplatin-induced NFκB expression and enhanced the NFκB-regulated Bax/Bcl-2 mRNA ratio. Cisplatin alone induced mRNA expression of excision repair cross complementation 1 (ERCC1) and thymidine phosphorylase (TP) through phosphorylation of ERK, p38 and PI3K/AKT pathways. However, fucoxanthin pretreatment significantly attenuated cisplatin-induced ERCC1 and TP mRNA expression, leading to improvement of chemotherapeutic efficacy of cisplatin. The results suggest that a combined treatment with fucoxanthin and cisplatin could lead to a potentially important new therapeutic strategy against human hepatoma cells.
Keywords: fucoxanthin; cisplatin; NFκB; DNA repair; MAPK; PI3K/AKT

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MDPI and ACS Style

Liu, C.-L.; Lim, Y.-P.; Hu, M.-L. Fucoxanthin Enhances Cisplatin-Induced Cytotoxicity via NFκB-Mediated Pathway and Downregulates DNA Repair Gene Expression in Human Hepatoma HepG2 Cells. Mar. Drugs 2013, 11, 50-66.

AMA Style

Liu C-L, Lim Y-P, Hu M-L. Fucoxanthin Enhances Cisplatin-Induced Cytotoxicity via NFκB-Mediated Pathway and Downregulates DNA Repair Gene Expression in Human Hepatoma HepG2 Cells. Marine Drugs. 2013; 11(1):50-66.

Chicago/Turabian Style

Liu, Cheng-Ling; Lim, Yun-Ping; Hu, Miao-Lin. 2013. "Fucoxanthin Enhances Cisplatin-Induced Cytotoxicity via NFκB-Mediated Pathway and Downregulates DNA Repair Gene Expression in Human Hepatoma HepG2 Cells." Mar. Drugs 11, no. 1: 50-66.

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