Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Mar. Drugs 2013, 11(1), 253-265; doi:10.3390/md11010253
Article

Quorum Sensing Inhibition by Asparagopsis taxiformis, a Marine Macro Alga: Separation of the Compound that Interrupts Bacterial Communication

1,* , 1
, 2
, 3
 and 2,4
Received: 29 November 2012; in revised form: 26 December 2012 / Accepted: 4 January 2013 / Published: 23 January 2013
(This article belongs to the Special Issue Marine Algae)
View Full-Text   |   Download PDF [679 KB, uploaded 23 January 2013]   |   Browse Figures
Abstract: The majority of the marine algal species, though completing their life cycle in seawater, are rarely susceptible to fouling, making them an important source of quorum sensing (QS) inhibitory substances. The separation and characterization of QS inhibitors are crucial for any potential application. Thirty marine macroalgae were tested for QS inhibition activity by using Chromobacterium violaceum CV026 as the reporter strain, and among them, Asparagopsis taxiformis showed antibacterial, as well as antiquorum, sensing activities. Cinnamaldehyde (75 mM) and methanol were used as positive and negative controls, respectively. The antiquorum sensing activity of A. taxiformis was further confirmed using the sensor strain, Serratia liquefaciens MG44, having green fluorescent protein (gfp). Methanolic extract of the alga was fractionated by solid phase extraction (SPE), and each fraction was tested for QS inhibition. Two types of activities were observed—zone of clearance (antibacterial activity) and zone of inhibition with or without finger-like projections (QS inhibition). Out of five SPE cartridges, Bond Elut PH showed clear separation of these two fractions. The Ion Cyclotron Resonance Fourier Transformation Mass Spectrometer (ICR-FT/MS) analysis of the fractions further supported the bioassay results. The presence of strong QS inhibitory compound in A. taxiformis indicates its potential use in antifouling preparations.
Keywords: marine algae; quorum sensing inhibitor; acyl homoserine lactone (AHL); Chromobacterium violaceum; solid phase extraction marine algae; quorum sensing inhibitor; acyl homoserine lactone (AHL); Chromobacterium violaceum; solid phase extraction
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Jha, B.; Kavita, K.; Westphal, J.; Hartmann, A.; Schmitt-Kopplin, P. Quorum Sensing Inhibition by Asparagopsis taxiformis, a Marine Macro Alga: Separation of the Compound that Interrupts Bacterial Communication. Mar. Drugs 2013, 11, 253-265.

AMA Style

Jha B, Kavita K, Westphal J, Hartmann A, Schmitt-Kopplin P. Quorum Sensing Inhibition by Asparagopsis taxiformis, a Marine Macro Alga: Separation of the Compound that Interrupts Bacterial Communication. Marine Drugs. 2013; 11(1):253-265.

Chicago/Turabian Style

Jha, Bhavanath; Kavita, Kumari; Westphal, Jenny; Hartmann, Anton; Schmitt-Kopplin, Philippe. 2013. "Quorum Sensing Inhibition by Asparagopsis taxiformis, a Marine Macro Alga: Separation of the Compound that Interrupts Bacterial Communication." Mar. Drugs 11, no. 1: 253-265.



Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert