Mar. Drugs 2013, 11(1), 266-273; doi:10.3390/md11010266
Article

Isolation and Purification of a Peptide from Bullacta exarata and Its Impaction of Apoptosis on Prostate Cancer Cell

School of Food Science and Pharmacy, Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products, Zhejiang Ocean University, Zhoushan 316000, China
* Author to whom correspondence should be addressed.
Received: 6 November 2012; in revised form: 20 December 2012 / Accepted: 24 December 2012 / Published: 23 January 2013
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Abstract: Bullacta exarata was hydrolyzed with trypsin to prepare peptides; Hydrolysates were isolated by ultrafiltration and purified using G-25 gel filtration. The purity of the Bullacta exarata was demonstrated by HPLC and its peptide sequence analysis was detected. The effects of BEPT II and BEPT II-1 on the proliferation of PC-3 cells were examined using a MTT assay. BEPT II and BEPT II-1 significantly inhibited the proliferation of PC-3 cells in a time- and dose-dependent manner. Annexin V/PI double staining studies showed exposing PC-3 cells to 5, or 15 mg/mL BEPT II-1 for 24 h increased the percentage of the early stage of apoptotic cells from 11.22% to 22.09%. In addition, typical morphologic changes were observed in the cells with acridine orange/ethidium bromide staining. These data support that BEPT II-1 has anticancer properties and merits further investigation to understand the mechanisms of BEPT II-1-induced apoptosis in PC-3 cells.
Keywords: Bullacta exarata peptides; isolation and purification; prostate cancer; apoptosis

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MDPI and ACS Style

Ma, J.; Huang, F.; Lin, H.; Wang, X. Isolation and Purification of a Peptide from Bullacta exarata and Its Impaction of Apoptosis on Prostate Cancer Cell. Mar. Drugs 2013, 11, 266-273.

AMA Style

Ma J, Huang F, Lin H, Wang X. Isolation and Purification of a Peptide from Bullacta exarata and Its Impaction of Apoptosis on Prostate Cancer Cell. Marine Drugs. 2013; 11(1):266-273.

Chicago/Turabian Style

Ma, Jianyin; Huang, Fangfang; Lin, Huanle; Wang, Xian. 2013. "Isolation and Purification of a Peptide from Bullacta exarata and Its Impaction of Apoptosis on Prostate Cancer Cell." Mar. Drugs 11, no. 1: 266-273.

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