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Mar. Drugs 2013, 11(1), 266-273; doi:10.3390/md11010266
Article

Isolation and Purification of a Peptide from Bullacta exarata and Its Impaction of Apoptosis on Prostate Cancer Cell

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,
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School of Food Science and Pharmacy, Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products, Zhejiang Ocean University, Zhoushan 316000, China
* Author to whom correspondence should be addressed.
Received: 6 November 2012 / Revised: 20 December 2012 / Accepted: 24 December 2012 / Published: 23 January 2013
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Abstract

Bullacta exarata was hydrolyzed with trypsin to prepare peptides; Hydrolysates were isolated by ultrafiltration and purified using G-25 gel filtration. The purity of the Bullacta exarata was demonstrated by HPLC and its peptide sequence analysis was detected. The effects of BEPT II and BEPT II-1 on the proliferation of PC-3 cells were examined using a MTT assay. BEPT II and BEPT II-1 significantly inhibited the proliferation of PC-3 cells in a time- and dose-dependent manner. Annexin V/PI double staining studies showed exposing PC-3 cells to 5, or 15 mg/mL BEPT II-1 for 24 h increased the percentage of the early stage of apoptotic cells from 11.22% to 22.09%. In addition, typical morphologic changes were observed in the cells with acridine orange/ethidium bromide staining. These data support that BEPT II-1 has anticancer properties and merits further investigation to understand the mechanisms of BEPT II-1-induced apoptosis in PC-3 cells.
Keywords: Bullacta exarata peptides; isolation and purification; prostate cancer; apoptosis Bullacta exarata peptides; isolation and purification; prostate cancer; apoptosis
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Ma, J.; Huang, F.; Lin, H.; Wang, X. Isolation and Purification of a Peptide from Bullacta exarata and Its Impaction of Apoptosis on Prostate Cancer Cell. Mar. Drugs 2013, 11, 266-273.

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