Mar. Drugs 2012, 10(1), 84-105; doi:10.3390/md10010084
Article

Tasco®: A Product of Ascophyllum nodosum Enhances Immune Response of Caenorhabditis elegans Against Pseudomonas aeruginosa Infection

1 Department of Environmental Sciences, Nova Scotia Agricultural College, P.O. Box 550, Truro, NS, B2B 5E3, Canada 2 Genome Research Chair Unit, Department of Biochemistry, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia 3 Acadian Seaplants Limited, 30 Brown Avenue, Dartmouth, NS, B3B 1X8, Canada
* Author to whom correspondence should be addressed.
Received: 28 November 2011; in revised form: 27 December 2011 / Accepted: 5 January 2012 / Published: 11 January 2012
(This article belongs to the Special Issue Marine Anti-infective Agents)
PDF Full-text Download PDF Full-Text [533 KB, uploaded 11 January 2012 15:18 CET]
Abstract: The effects of Tasco®, a product made from the brown seaweed (Ascophyllum nodosum) were tested for the ability to protect Caenorhabditis elegans against Pseudomonas aeruginosa infection. A water extract of Tasco® (TWE) reduced P. aeruginosa inflicted mortality in the nematode. The TWE, at a concentration of 300 µg/mL, offered the maximum protection and induced the expression of innate immune response genes viz.; zk6.7 (Lypases), lys-1 (Lysozyme), spp-1 (Saponin like protein), f28d1.3 (Thaumatin like protein), t20g5.7 (Matridin SK domain protein), abf-1 (Antibacterial protein) and f38a1.5 (Lectin family protein). Further, TWE treatment also affected a number of virulence components of the P. aeuroginosa and reduced its secreted virulence factors such as lipase, proteases and toxic metabolites; hydrogen cyanide and pyocyanin. Decreased virulence factors were associated with a significant reduction in expression of regulatory genes involved in quorum sensing, lasI, lasR, rhlI and rhlR. In conclusion, the TWE-treatment protected the C. elegans against P. aeruginosa infection by a combination of effects on the innate immunity of the worms and direct effects on the bacterial quorum sensing and virulence factors.
Keywords: Tasco®; Ascophyllum nodosum; Caenorhabiditis elegans; Pseudomonas aeruginosa; virulence factors; innate immunity; gene expression; quorum sensing

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Kandasamy, S.; Khan, W.; Evans, F.; Critchley, A.T.; Prithiviraj, B. Tasco®: A Product of Ascophyllum nodosum Enhances Immune Response of Caenorhabditis elegans Against Pseudomonas aeruginosa Infection. Mar. Drugs 2012, 10, 84-105.

AMA Style

Kandasamy S, Khan W, Evans F, Critchley AT, Prithiviraj B. Tasco®: A Product of Ascophyllum nodosum Enhances Immune Response of Caenorhabditis elegans Against Pseudomonas aeruginosa Infection. Marine Drugs. 2012; 10(1):84-105.

Chicago/Turabian Style

Kandasamy, Saveetha; Khan, Wajahatullah; Evans, Franklin; Critchley, Alan T.; Prithiviraj, Balakrishnan. 2012. "Tasco®: A Product of Ascophyllum nodosum Enhances Immune Response of Caenorhabditis elegans Against Pseudomonas aeruginosa Infection." Mar. Drugs 10, no. 1: 84-105.

Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert