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Mar. Drugs 2012, 10(1), 84-105; doi:10.3390/md10010084

Tasco®: A Product of Ascophyllum nodosum Enhances Immune Response of Caenorhabditis elegans Against Pseudomonas aeruginosa Infection

1
Department of Environmental Sciences, Nova Scotia Agricultural College, P.O. Box 550, Truro, NS, B2B 5E3, Canada
2
Genome Research Chair Unit, Department of Biochemistry, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia
3
Acadian Seaplants Limited, 30 Brown Avenue, Dartmouth, NS, B3B 1X8, Canada
*
Author to whom correspondence should be addressed.
Received: 28 November 2011 / Revised: 27 December 2011 / Accepted: 5 January 2012 / Published: 11 January 2012
(This article belongs to the Special Issue Marine Anti-infective Agents)
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Abstract

The effects of Tasco®, a product made from the brown seaweed (Ascophyllum nodosum) were tested for the ability to protect Caenorhabditis elegans against Pseudomonas aeruginosa infection. A water extract of Tasco® (TWE) reduced P. aeruginosa inflicted mortality in the nematode. The TWE, at a concentration of 300 µg/mL, offered the maximum protection and induced the expression of innate immune response genes viz.; zk6.7 (Lypases), lys-1 (Lysozyme), spp-1 (Saponin like protein), f28d1.3 (Thaumatin like protein), t20g5.7 (Matridin SK domain protein), abf-1 (Antibacterial protein) and f38a1.5 (Lectin family protein). Further, TWE treatment also affected a number of virulence components of the P. aeuroginosa and reduced its secreted virulence factors such as lipase, proteases and toxic metabolites; hydrogen cyanide and pyocyanin. Decreased virulence factors were associated with a significant reduction in expression of regulatory genes involved in quorum sensing, lasI, lasR, rhlI and rhlR. In conclusion, the TWE-treatment protected the C. elegans against P. aeruginosa infection by a combination of effects on the innate immunity of the worms and direct effects on the bacterial quorum sensing and virulence factors. View Full-Text
Keywords: Tasco®; Ascophyllum nodosum; Caenorhabiditis elegans; Pseudomonas aeruginosa; virulence factors; innate immunity; gene expression; quorum sensing Tasco®; Ascophyllum nodosum; Caenorhabiditis elegans; Pseudomonas aeruginosa; virulence factors; innate immunity; gene expression; quorum sensing
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Kandasamy, S.; Khan, W.; Evans, F.; Critchley, A.T.; Prithiviraj, B. Tasco®: A Product of Ascophyllum nodosum Enhances Immune Response of Caenorhabditis elegans Against Pseudomonas aeruginosa Infection. Mar. Drugs 2012, 10, 84-105.

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