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Detecting Proteins in Highly Autofluorescent Cells Using Quantum Dot Antibody Conjugates
Department of Marine Science, University of Southern Mississippi, 1020 Balch Blvd., Stennis Space Center, MS 39529, USA
* Author to whom correspondence should be addressed.
Received: 3 August 2009; in revised form: 10 September 2009 / Accepted: 22 September 2009 / Published: 23 September 2009
Abstract: We have applied quantum dot (Qdot) antibody conjugates as a biomolecular probe for cellular proteins important in biogeochemical cycling in the sea. Conventional immunological methods have been hampered by the strong autofluorescence found in cyanobacteria cells. Qdot conjugates provide an ideal alternative for studies that require long-term imaging of cells such as detection of low abundance cellular antigens by fluorescence microscopy. The advantage of Qdot labeled probes over conventional immunological methods is the photostability of the probe. Phycoerythrin bleaches in cyanobacterial cells under prolonged UV or blue light excitation, which means that the semiconducting nanocrystal probe, the Qdot, can yield a strong fluorescent signal without interference from cellular pigments.
Keywords: quantum dot; autofluorescent cells; nitrogen fixation; iron stress; cyanobacteria
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MDPI and ACS Style
Orcutt, K.M.; Ren, S.; Gundersen, K. Detecting Proteins in Highly Autofluorescent Cells Using Quantum Dot Antibody Conjugates. Sensors 2009, 9, 7540-7549.
Orcutt KM, Ren S, Gundersen K. Detecting Proteins in Highly Autofluorescent Cells Using Quantum Dot Antibody Conjugates. Sensors. 2009; 9(9):7540-7549.
Orcutt, Karen M.; Ren, Shanshan; Gundersen, Kjell. 2009. "Detecting Proteins in Highly Autofluorescent Cells Using Quantum Dot Antibody Conjugates." Sensors 9, no. 9: 7540-7549.