Sensors 2009, 9(9), 7540-7549; doi:10.3390/s90907540
Article

Detecting Proteins in Highly Autofluorescent Cells Using Quantum Dot Antibody Conjugates

Department of Marine Science, University of Southern Mississippi, 1020 Balch Blvd., Stennis Space Center, MS 39529, USA
* Author to whom correspondence should be addressed.
Received: 3 August 2009; in revised form: 10 September 2009 / Accepted: 22 September 2009 / Published: 23 September 2009
(This article belongs to the Special Issue Nanotechnological Advances in Biosensors)
PDF Full-text Download PDF Full-Text [1302 KB, uploaded 23 September 2009 05:16 CEST]
Abstract: We have applied quantum dot (Qdot) antibody conjugates as a biomolecular probe for cellular proteins important in biogeochemical cycling in the sea. Conventional immunological methods have been hampered by the strong autofluorescence found in cyanobacteria cells. Qdot conjugates provide an ideal alternative for studies that require long-term imaging of cells such as detection of low abundance cellular antigens by fluorescence microscopy. The advantage of Qdot labeled probes over conventional immunological methods is the photostability of the probe. Phycoerythrin bleaches in cyanobacterial cells under prolonged UV or blue light excitation, which means that the semiconducting nanocrystal probe, the Qdot, can yield a strong fluorescent signal without interference from cellular pigments.
Keywords: quantum dot; autofluorescent cells; nitrogen fixation; iron stress; cyanobacteria

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Orcutt, K.M.; Ren, S.; Gundersen, K. Detecting Proteins in Highly Autofluorescent Cells Using Quantum Dot Antibody Conjugates. Sensors 2009, 9, 7540-7549.

AMA Style

Orcutt KM, Ren S, Gundersen K. Detecting Proteins in Highly Autofluorescent Cells Using Quantum Dot Antibody Conjugates. Sensors. 2009; 9(9):7540-7549.

Chicago/Turabian Style

Orcutt, Karen M.; Ren, Shanshan; Gundersen, Kjell. 2009. "Detecting Proteins in Highly Autofluorescent Cells Using Quantum Dot Antibody Conjugates." Sensors 9, no. 9: 7540-7549.

Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert