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Sensors 2009, 9(9), 7540-7549; doi:10.3390/s90907540
Article

Detecting Proteins in Highly Autofluorescent Cells Using Quantum Dot Antibody Conjugates

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Received: 3 August 2009; in revised form: 10 September 2009 / Accepted: 22 September 2009 / Published: 23 September 2009
(This article belongs to the Special Issue Nanotechnological Advances in Biosensors)
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Abstract: We have applied quantum dot (Qdot) antibody conjugates as a biomolecular probe for cellular proteins important in biogeochemical cycling in the sea. Conventional immunological methods have been hampered by the strong autofluorescence found in cyanobacteria cells. Qdot conjugates provide an ideal alternative for studies that require long-term imaging of cells such as detection of low abundance cellular antigens by fluorescence microscopy. The advantage of Qdot labeled probes over conventional immunological methods is the photostability of the probe. Phycoerythrin bleaches in cyanobacterial cells under prolonged UV or blue light excitation, which means that the semiconducting nanocrystal probe, the Qdot, can yield a strong fluorescent signal without interference from cellular pigments.
Keywords: quantum dot; autofluorescent cells; nitrogen fixation; iron stress; cyanobacteria quantum dot; autofluorescent cells; nitrogen fixation; iron stress; cyanobacteria
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Orcutt, K.M.; Ren, S.; Gundersen, K. Detecting Proteins in Highly Autofluorescent Cells Using Quantum Dot Antibody Conjugates. Sensors 2009, 9, 7540-7549.

AMA Style

Orcutt KM, Ren S, Gundersen K. Detecting Proteins in Highly Autofluorescent Cells Using Quantum Dot Antibody Conjugates. Sensors. 2009; 9(9):7540-7549.

Chicago/Turabian Style

Orcutt, Karen M.; Ren, Shanshan; Gundersen, Kjell. 2009. "Detecting Proteins in Highly Autofluorescent Cells Using Quantum Dot Antibody Conjugates." Sensors 9, no. 9: 7540-7549.


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