Evanescent Wave Fiber Optic Biosensor for Salmonella Detection in Food
AbstractSalmonella enterica is a major food-borne pathogen of world-wide concern. Sensitive and rapid detection methods to assess product safety before retail distribution are highly desirable. Since Salmonella is most commonly associated with poultry products, an evanescent wave fiber-optic assay was developed to detect Salmonella in shell egg and chicken breast and data were compared with a time-resolved fluorescence (TRF) assay. Anti-Salmonella polyclonal antibody was immobilized onto the surface of an optical fiber using biotin-avidin interactions to capture Salmonella. Alexa Fluor 647-conjugated antibody (MAb 2F-11) was used as the reporter. Detection occurred when an evanescent wave from a laser (635 nm) excited the Alexa Fluor and the fluorescence was measured by a laser-spectrofluorometer at 710 nm. The biosensor was specific for Salmonella and the limit of detection was established to be 103 cfu/mL in pure culture and 104 cfu/mL with egg and chicken breast samples when spiked with 102 cfu/mL after 2–6 h of enrichment. The results indicate that the performance of the fiber-optic sensor is comparable to TRF, and can be completed in less than 8 h, providing an alternative to the current detection methods. View Full-Text
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Valadez, A.M.; Lana, C.A.; Tu, S.-I.; Morgan, M.T.; Bhunia, A.K. Evanescent Wave Fiber Optic Biosensor for Salmonella Detection in Food. Sensors 2009, 9, 5810-5824.
Valadez AM, Lana CA, Tu S-I, Morgan MT, Bhunia AK. Evanescent Wave Fiber Optic Biosensor for Salmonella Detection in Food. Sensors. 2009; 9(7):5810-5824.Chicago/Turabian Style
Valadez, Angela M.; Lana, Carlos A.; Tu, Shu-I; Morgan, Mark T.; Bhunia, Arun K. 2009. "Evanescent Wave Fiber Optic Biosensor for Salmonella Detection in Food." Sensors 9, no. 7: 5810-5824.