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Sensors 2009, 9(7), 5810-5824; https://doi.org/10.3390/s90705810

Evanescent Wave Fiber Optic Biosensor for Salmonella Detection in Food

1
Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, Indiana 47907, USA
2
School of Aeronautics & Astronautics, Purdue University, 701 W. Stadium Ave., West Lafayette, Indiana 47907, USA
3
United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA
*
Author to whom correspondence should be addressed.
Received: 8 May 2009 / Revised: 15 July 2009 / Accepted: 17 July 2009 / Published: 21 July 2009
(This article belongs to the Section Biosensors)
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Abstract

Salmonella enterica is a major food-borne pathogen of world-wide concern. Sensitive and rapid detection methods to assess product safety before retail distribution are highly desirable. Since Salmonella is most commonly associated with poultry products, an evanescent wave fiber-optic assay was developed to detect Salmonella in shell egg and chicken breast and data were compared with a time-resolved fluorescence (TRF) assay. Anti-Salmonella polyclonal antibody was immobilized onto the surface of an optical fiber using biotin-avidin interactions to capture Salmonella. Alexa Fluor 647-conjugated antibody (MAb 2F-11) was used as the reporter. Detection occurred when an evanescent wave from a laser (635 nm) excited the Alexa Fluor and the fluorescence was measured by a laser-spectrofluorometer at 710 nm. The biosensor was specific for Salmonella and the limit of detection was established to be 103 cfu/mL in pure culture and 104 cfu/mL with egg and chicken breast samples when spiked with 102 cfu/mL after 2–6 h of enrichment. The results indicate that the performance of the fiber-optic sensor is comparable to TRF, and can be completed in less than 8 h, providing an alternative to the current detection methods. View Full-Text
Keywords: Salmonella; fiber optic sensor; time–resolved fluorescence assay; egg; chicken Salmonella; fiber optic sensor; time–resolved fluorescence assay; egg; chicken
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Valadez, A.M.; Lana, C.A.; Tu, S.-I.; Morgan, M.T.; Bhunia, A.K. Evanescent Wave Fiber Optic Biosensor for Salmonella Detection in Food. Sensors 2009, 9, 5810-5824.

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