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Sensors 2009, 9(5), 3713-3744; doi:10.3390/s90503713

Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems

1,* , 2
1 Department of Biomedical Engineering / Cornell University, 317 Stocking Hall, Ithaca, NY 14853, USA 2 College of Nanoscale Science and Engineering / University at Albany State University of New York, 255 Fuller Rd., Albany, NY 12203, USA 3 Department of Food Science / Cornell University, 312 Stocking Hall, Ithaca, NY 14853, USA
* Author to whom correspondence should be addressed.
Received: 9 April 2009 / Revised: 12 May 2009 / Accepted: 18 May 2009 / Published: 18 May 2009
(This article belongs to the Special Issue Pathogen Sensors)
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The advent of nucleic acid-based pathogen detection methods offers increased sensitivity and specificity over traditional microbiological techniques, driving the development of portable, integrated biosensors. The miniaturization and automation of integrated detection systems presents a significant advantage for rapid, portable field-based testing. In this review, we highlight current developments and directions in nucleic acid-based micro total analysis systems for the detection of bacterial pathogens. Recent progress in the miniaturization of microfluidic processing steps for cell capture, DNA extraction and purification, polymerase chain reaction, and product detection are detailed. Discussions include strategies and challenges for implementation of an integrated portable platform.
Keywords: pathogen; sensors; biosensors; PCR; DNA; microfluidics; μTAS; lab-on-a-chip pathogen; sensors; biosensors; PCR; DNA; microfluidics; μTAS; lab-on-a-chip
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Lui, C.; Cady, N.C.; Batt, C.A. Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems. Sensors 2009, 9, 3713-3744.

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