Next Article in Journal
Performance Analysis of the SIFT Operator for Automatic Feature Extraction and Matching in Photogrammetric Applications
Next Article in Special Issue
Antibody-Based Sensors: Principles, Problems and Potential for Detection of Pathogens and Associated Toxins
Previous Article in Journal
A Hybrid Stochastic Approach for Self-Location of Wireless Sensors in Indoor Environments
Previous Article in Special Issue
Advances in Microbial Biofilm Prevention on Indwelling Medical Devices with Emphasis on Usage of Acoustic Energy
Sensors 2009, 9(5), 3713-3744; doi:10.3390/s90503713
Review

Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems

1,* , 2
 and 3
1 Department of Biomedical Engineering / Cornell University, 317 Stocking Hall, Ithaca, NY 14853, USA 2 College of Nanoscale Science and Engineering / University at Albany State University of New York, 255 Fuller Rd., Albany, NY 12203, USA 3 Department of Food Science / Cornell University, 312 Stocking Hall, Ithaca, NY 14853, USA
* Author to whom correspondence should be addressed.
Received: 9 April 2009 / Revised: 12 May 2009 / Accepted: 18 May 2009 / Published: 18 May 2009
(This article belongs to the Special Issue Pathogen Sensors)
View Full-Text   |   Download PDF [1090 KB, uploaded 21 June 2014]   |   Browse Figures

Abstract

The advent of nucleic acid-based pathogen detection methods offers increased sensitivity and specificity over traditional microbiological techniques, driving the development of portable, integrated biosensors. The miniaturization and automation of integrated detection systems presents a significant advantage for rapid, portable field-based testing. In this review, we highlight current developments and directions in nucleic acid-based micro total analysis systems for the detection of bacterial pathogens. Recent progress in the miniaturization of microfluidic processing steps for cell capture, DNA extraction and purification, polymerase chain reaction, and product detection are detailed. Discussions include strategies and challenges for implementation of an integrated portable platform.
Keywords: pathogen; sensors; biosensors; PCR; DNA; microfluidics; μTAS; lab-on-a-chip pathogen; sensors; biosensors; PCR; DNA; microfluidics; μTAS; lab-on-a-chip
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

Lui, C.; Cady, N.C.; Batt, C.A. Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems. Sensors 2009, 9, 3713-3744.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert