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Sensors 2009, 9(3), 1355-1369; doi:10.3390/s90301355
Article

Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka´s reaction and Quartz Crystal Microbalance

1, 1, 1, 1, 2, 3, 3, 3, 5, 6, 2 and 1,*
1 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic 2 Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic 3 Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic 4 Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Udolni 53, CZ-602 00 Brno, Czech Republic 5 Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic 6 Tomas Bata University, T.G. Masaryka 275, CZ-762 72 Zlin, Czech Republic
* Author to whom correspondence should be addressed.
Received: 8 October 2008 / Revised: 14 February 2009 / Accepted: 17 February 2009 / Published: 26 February 2009
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Abstract

Treatment strategies for tumour diseases are progressively focusing on personalization of medicine. However, this focus requires methods revealing the early general biological mechanisms, including the formation anti-cancer drugs’ resistance. The low molecular mass protein metallothionein is thought to be the crucial for the formation of resistance in tumour treatment based on the platinum-cytostatics. The interactions between metallothionein (MT) and cisplatin were determined by the adsorptive transfer stripping technique coupled with the differential pulse votlammetry Brdicka´s reaction. The signals related to the MT-cisplatin complex appeared at -0.9 V. The formation of this complex depended on the time of interaction between cisplatin and MT. The complex formation was consequently confirmed by quartz crystal microbalance analyses. The formation of this complex was detectable even after a 20 s long interaction. Moreover, we detected presence of MT-cisplatin complex in the blood of male rats treated with this drug.
Keywords: Cancer; Metallothionein; Cisplatin; Protein-Drug Interaction; Voltammetry; Brdicka´s reaction; Quartz Crystal Microbalance Cancer; Metallothionein; Cisplatin; Protein-Drug Interaction; Voltammetry; Brdicka´s reaction; Quartz Crystal Microbalance
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Huska, D.; Fabrik, I.; Baloun, J.; Adam, V.; Masarik, M.; Hubalek, J.; Vasku, A.; Trnkova, L.; Horna, A.; Zeman, L.; Kizek, R. Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka´s reaction and Quartz Crystal Microbalance. Sensors 2009, 9, 1355-1369.

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