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• Al-Ahmed, A
• Ndangili, PM.
• Jahed, N
• Baker, PL.
• Iwuoha, EI.
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• Al-Ahmed, A
• Ndangili, PM.
• Jahed, N
• Baker, PL.
• Iwuoha, EI.
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• Al-Ahmed, A
• Ndangili, PM.
• Jahed, N
• Baker, PL.
• Iwuoha, EI.

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Sensors 2009, 9(12), 9965-9976; doi:10.3390/s91209965

Article

Polyester Sulphonic Acid Interstitial Nanocomposite Platform for Peroxide Biosensor

Amir Al-Ahmed, Peter M. Ndangili, Nazeem Jahed, Priscilla G. L. Baker*  and Emmanuel I. Iwuoha

SensorLab, Department of Chemistry, University of Western Cape, Bellville, 7535, Cape Town, South Africa

* Author to whom correspondence should be addressed.

Received: 16 November 2009; in revised form: 25 November 2009 / Accepted: 7 December 2009 / Published: 8 December 2009

(This article belongs to the Section Biosensors)

Download PDF Full-Text [419 KB, uploaded 9 December 2009 09:49 CET]

Abstract: A novel enzyme immobilization platform was prepared on a platinum disk working electrode by polymerizing aniline inside the interstitial pores of polyester sulphonic acid sodium salt (PESA). Scanning electron microscopy study showed the formation of homogeneous sulphonated polyaniline (PANI) nanotubes (~90 nm) and thermogravimetric analysis (TGA) confirmed that the nanotubes were stable up to 230 °C. The PANI:PESA nanocomposite showed a quasi-reversible redox behaviour in phosphate buffer saline. Horseradish peroxidase (HRP) was immobilized on to this modified electrode for hydrogen peroxide detection. The biosensor gave a sensitivity of 1.33 μA (μM)^-1 and a detection limit of 0.185 μM for H₂O₂. Stability experiments showed that the biosensor retained more than 64% of its initial sensitivity over four days of storage at 4 °C.

Keywords: peroxide biosensors; immobilization of enzyme; HRP; electrochemical polymerisation; polyaniline nanotubes; polyester sulphonic acid

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