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Flow Injection Chemiluminescent Immunoassay for Carcinoembryonic Antigen Using Boronic Immunoaffinity Column
Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, 210004, China
Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Environmental Science, Nanjing Normal University, Nanjing, 210097, China
* Author to whom correspondence should be addressed.
Received: 3 November 2009; in revised form: 11 December 2009 / Accepted: 15 December 2009 / Published: 21 December 2009
Abstract: A flow injection chemiluminescence immunoassay for rapid and sensitive detection of carcinoembryonic antigen (CEA) by using a phenylboronic acid-based immunoaffinity column as a glycoprotein collector was proposed in this paper. The column was prepared by coupling of 3-aminophenylboronic acid on the glass beads through a γ-glycidoxypropyltrimethoxysilane (GPMS) linkage. Based on an indirect competitive immunoreaction, the mixture of CEA sample and enzyme conjugated CEA antibody (HRP-anti-CEA) was incubated in advance, followed by direct injection to the column to capture free HRP-labeled CEA antibody in the column. The trapped HRP-labeled antibody was detected by flow inject chemiluminescence in the presence of luminol and hydrogen peroxide. The decreased chemiluminescent signal was proportional to the concentration of CEA in the range of 3.0–30.0 ng/mL with a correlation coefficient of 0.998. The column showed an acceptable reproducibility and stability and is potentially used for practical clinical detection of the serum CEA level.
Keywords: flow injection chemiluminescence immunoassay; carcinoembryonic antigen; boronic immunoaffinity column
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MDPI and ACS Style
Ma, X.; Li, H.; Wu, M.; Sun, C.; Li, L.; Yang, X. Flow Injection Chemiluminescent Immunoassay for Carcinoembryonic Antigen Using Boronic Immunoaffinity Column. Sensors 2009, 9, 10389-10399.
Ma X, Li H, Wu M, Sun C, Li L, Yang X. Flow Injection Chemiluminescent Immunoassay for Carcinoembryonic Antigen Using Boronic Immunoaffinity Column. Sensors. 2009; 9(12):10389-10399.
Ma, Xiaoling; Li, Huihui; Wu, Min; Sun, Chong; Li, Laifa; Yang, Xiaodi. 2009. "Flow Injection Chemiluminescent Immunoassay for Carcinoembryonic Antigen Using Boronic Immunoaffinity Column." Sensors 9, no. 12: 10389-10399.