Visible Genotype Sensor Array
AbstractA visible sensor array system for simultaneous multiple SNP genotyping has been developed using a new plastic base with specific surface chemistry. Discrimination of SNP alleles is carried out by an allele-specific extension reaction using immobilized oligonucleotide primers. The 3’-ends of oligonucleotide primers are modified with a locked nucleic acid to enhance their efficiency in allelic discrimination. Biotin-dUTPs included in the reaction mixture are selectively incorporated into extending primer sequences and are utilized as tags for alkaline phosphatase-mediated precipitation of colored chemical substrates onto the surface of the plastic base. The visible precipitates allow immediate inspection of typing results by the naked eye and easy recording by a digital camera equipped on a commercial mobile phone. Up to four individuals can be analyzed on a single sensor array and multiple sensor arrays can be handled in a single operation. All of the reactions can be performed within one hour using conventional laboratory instruments. This visible genotype sensor array is suitable for “focused genomics” that follows “comprehensive genomics”.
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Michikawa, Y.; Suga, T.; Ohtsuka, Y.; Matsumoto, I.; Ishikawa, A.; Ishikawa, K.; Iwakawa, M.; Imai, T. Visible Genotype Sensor Array. Sensors 2008, 8, 2722-2735.
Michikawa Y, Suga T, Ohtsuka Y, Matsumoto I, Ishikawa A, Ishikawa K, Iwakawa M, Imai T. Visible Genotype Sensor Array. Sensors. 2008; 8(4):2722-2735.Chicago/Turabian Style
Michikawa, Yuichi; Suga, Tomo; Ohtsuka, Yoshimi; Matsumoto, Izumi; Ishikawa, Atsuko; Ishikawa, Kenichi; Iwakawa, Mayumi; Imai, Takashi. 2008. "Visible Genotype Sensor Array." Sensors 8, no. 4: 2722-2735.