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• Michikawa, Y
• Suga, T
• Ohtsuka, Y
• Matsumoto, I
• Ishikawa, A
• Ishikawa, K
• Iwakawa, M
• Imai, T
• [+] on Google Scholar
• Michikawa, Y
• Suga, T
• Ohtsuka, Y
• Matsumoto, I
• Ishikawa, A
• Ishikawa, K
• Iwakawa, M
• Imai, T
• [+] on PubMed
• Michikawa, Y
• Suga, T
• Ohtsuka, Y
• Matsumoto, I
• Ishikawa, A
• Ishikawa, K
• Iwakawa, M
• Imai, T

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Sensors 2008, 8(4), 2722-2735; doi:10.3390/s8042722

Review

Visible Genotype Sensor Array

Yuichi Michikawa* , Tomo Suga, Yoshimi Ohtsuka, Izumi Matsumoto, Atsuko Ishikawa, Kenichi Ishikawa, Mayumi Iwakawa and Takashi Imai

RadGenomics Project, Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, Chiba, 263-8555, Japan

* Author to whom correspondence should be addressed.

Received: 25 January 2008 / Accepted: 15 April 2008 / Published: 17 April 2008

(This article belongs to the Special Issue Bioanalysis in Vivo/in Vitro)

Download PDF Full-Text [422 KB, uploaded 16 September 2008 11:02 CEST]

Abstract: A visible sensor array system for simultaneous multiple SNP genotyping has been developed using a new plastic base with specific surface chemistry. Discrimination of SNP alleles is carried out by an allele-specific extension reaction using immobilized oligonucleotide primers. The 3’-ends of oligonucleotide primers are modified with a locked nucleic acid to enhance their efficiency in allelic discrimination. Biotin-dUTPs included in the reaction mixture are selectively incorporated into extending primer sequences and are utilized as tags for alkaline phosphatase-mediated precipitation of colored chemical substrates onto the surface of the plastic base. The visible precipitates allow immediate inspection of typing results by the naked eye and easy recording by a digital camera equipped on a commercial mobile phone. Up to four individuals can be analyzed on a single sensor array and multiple sensor arrays can be handled in a single operation. All of the reactions can be performed within one hour using conventional laboratory instruments. This visible genotype sensor array is suitable for “focused genomics” that follows “comprehensive genomics”.

Keywords: Visible sensor; SNP; array; plastic; primer extension.

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