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DNA Extraction Systematics for Spectroscopic Studies
Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba, Av. Shishima Hifumi, 2911, 12244-000, São José dos Campos, São Paulo, Brazil
Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Catequese 242, 09090-400, Santo André, São Paulo, Brazil
* Author to whom correspondence should be addressed.
Received: 29 April 2008; in revised form: 19 May 2008 / Accepted: 20 May 2008 / Published: 1 June 2008
Abstract: Study of genetic material allows the comprehension the origin of the many biochemical changes that follow diseases, like cancer, promoting the development of early preventive inquiry and more efficient individual treatments. Raman spectroscopy can be an important tool in DNA study, since it allows probe molecular vibrations of genetic material in a fast way. The present work established a systematic way for extract DNA in suitable concentrations and structural integrity allowing studies by Raman spectroscopy or other spectroscopic technique, including bio-analytical sensors for probing genetic alterations.
Keywords: DNA; Raman spectroscopy; Cancer.
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Palma, B.F.; Ferrari, A.B.; Bitar, R.A.; Cardoso, M.A.G.; Martin, A.A.; Da Silva Martinho, H. DNA Extraction Systematics for Spectroscopic Studies. Sensors 2008, 8, 3624-3632.
Palma BF, Ferrari AB, Bitar RA, Cardoso MAG, Martin AA, Da Silva Martinho H. DNA Extraction Systematics for Spectroscopic Studies. Sensors. 2008; 8(6):3624-3632.
Palma, Bianca F.; Ferrari, Amanda B.; Bitar, Renata A.; Cardoso, Maria A.G.; Martin, Airton A.; Da Silva Martinho, Herculano. 2008. "DNA Extraction Systematics for Spectroscopic Studies." Sensors 8, no. 6: 3624-3632.