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• Pan, D
• Zuo, X
• Wan, Y
• Wang, L
• Zhang, J
• Song, S
• Fan, C
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• Pan, D
• Zuo, X
• Wan, Y
• Wang, L
• Zhang, J
• Song, S
• Fan, C
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• Pan, D
• Zuo, X
• Wan, Y
• Wang, L
• Zhang, J
• Song, S
• Fan, C

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Sensors 2007, 7(11), 2671-2680; doi:10.3390/s7112671

Article

Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue

Dun Pan, Xiaolei Zuo, Ying Wan, Lihua Wang, Jiong Zhang, Shiping Song*  and Chunhai Fan

Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

* Author to whom correspondence should be addressed.

Received: 31 October 2007 / Accepted: 9 November 2007 / Published: 12 November 2007

(This article belongs to the Special Issue Utilization of Electrochemical Sensors and Biosensors in Biochemistry and Molecular Biology)

Download PDF Full-Text [642 KB, uploaded 20 June 2008 16:52 CEST]

Abstract: In this work, we reported a systematic investigation on the interactions betweenmethylene blue (MB) and surface-confined DNA by using electrochemical methods. Wedemonstrated that the redox potential of MB and binding and dissociation kinetics of MB toDNA differed significantly for single-stranded DNA (ss-DNA) and double-stranded DNA(ds-DNA) immobilized on gold electrodes. This was possibly due to the different bindingmechanism between MB and ss- or ds-DNA. This work might provide useful informationfor developing MB-based sequence-specific electrochemical DNA sensors.

Keywords: Electrochemical DNA sensors; methylene blue; electrochemical indicators.

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