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Sensors 2007, 7(11), 2825-2834; doi:10.3390/s7112825
Article
Piezoelectric Biosensor for a Simple Serological Diagnosis of Tularemia in Infected European Brown Hares (Lepus europaeus)
Miroslav Pohanka 1,*
, František Treml 2 , Martin Hubálek 3 , Hana Banďouchová 4 , Miroslava Beklová 4 and Jiří Pikula 4
, František Treml 2 , Martin Hubálek 3 , Hana Banďouchová 4 , Miroslava Beklová 4 and Jiří Pikula 4
1
Center of Biological Defense, Těchonín, Czech Republic
2
Department of Infectious Diseases and Epizootiology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic
3
Institute of Molecular Pathology, University of Defence, Hradec Králové, Czech Republic
4
Department of Veterinary Ecology and Environmental Protection, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic
* Author to whom correspondence should be addressed.
Received: 17 October 2007 / Accepted: 14 November 2007 / Published: 19 November 2007
(This article belongs to the Special Issue Utilization of Electrochemical Sensors and Biosensors in Biochemistry and Molecular Biology)
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Abstract: Piezoelectric biosensor was used for diagnosis of infection by Francisellatularensis subsp. holarctica in European brown hares. Two kinds of experiments wereperformed in this study. First, sera from experimentally infected European brown hares(Lepus europaeus) were assayed by piezoelectric biosensor and the seventh day postinfection was found as the first one when statistically significant diagnosis of tularemia waspossible; all other sera collected from hares later than on day 7 following the infection werefound tularemia positive. Typing to classify the field strain of F. tularensis used for theexperimental infection was confirmed by proteome study. Second, sera from 35 Europeanbrown hare specimens sampled at hunting grounds and tested as tularemia positive by slowagglutination allowed diagnosis of tularemia by the piezoelectric biosensor. All these sera ofnaturally infected hares were found as tularemia positive, too. Efficacy of the piezoelectricbiosensor for the serological diagnosis of tularemia is discussed.
Keywords: Francisella tularensis; zoonosis; reservoir host; experimental and natural infection; serology; immunosensor; agglutination test
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MDPI and ACS Style
Pohanka, M.; Treml, F.; Hubálek, M.; Banďouchová, H.; Beklová, M.; Pikula, J. Piezoelectric Biosensor for a Simple Serological Diagnosis of Tularemia in Infected European Brown Hares (Lepus europaeus). Sensors 2007, 7, 2825-2834.
AMA StylePohanka M, Treml F, Hubálek M, Banďouchová H, Beklová M, Pikula J. Piezoelectric Biosensor for a Simple Serological Diagnosis of Tularemia in Infected European Brown Hares (Lepus europaeus). Sensors. 2007; 7(11):2825-2834.
Chicago/Turabian StylePohanka, Miroslav; Treml, František; Hubálek, Martin; Banďouchová, Hana; Beklová, Miroslava; Pikula, Jiří. 2007. "Piezoelectric Biosensor for a Simple Serological Diagnosis of Tularemia in Infected European Brown Hares (Lepus europaeus)." Sensors 7, no. 11: 2825-2834.