Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Sensors 2007, 7(10), 2080-2095; doi:10.3390/s7102080
Article

Determination of DPPH Radical Oxidation Caused by Methanolic Extracts of Some Microalgal Species by Linear Regression Analysis of Spectrophotometric Measurements

1,* , 1, 2, 2, 1 and 3
Received: 20 June 2007; Accepted: 1 October 2007 / Published: 3 October 2007
(This article belongs to the Special Issue Physiological Sensing)
View Full-Text   |   Download PDF [237 KB, uploaded 21 June 2014]
Abstract: The demonstrated modified spectrophotometric method makes use of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and its specific absorbance properties. Theabsorbance decreases when the radical is reduced by antioxidants. In contrast to otherinvestigations, the absorbance was measured at a wavelength of 550 nm. This wavelengthenabled the measurements of the stable free DPPH radical without interference frommicroalgal pigments. This approach was applied to methanolic microalgae extracts for twodifferent DPPH concentrations. The changes in absorbance measured vs. the concentrationof the methanolic extract resulted in curves with a linear decrease ending in a saturationregion. Linear regression analysis of the linear part of DPPH reduction versus extractconcentration enabled the determination of the microalgae’s methanolic extractsantioxidative potentials which was independent to the employed DPPH concentrations. Theresulting slopes showed significant differences (6 - 34 μmol DPPH g-1 extractconcentration) between the single different species of microalgae (Anabaena sp.,Isochrysis galbana, Phaeodactylum tricornutum, Porphyridium purpureum, Synechocystissp. PCC6803) in their ability to reduce the DPPH radical. The independency of the signal on the DPPH concentration is a valuable advantage over the determination of the EC50 value.
Keywords: absorbance decrease; antioxidative potential; microalgae; radical scavenging; linear regression analysis; spectrophotometric approach absorbance decrease; antioxidative potential; microalgae; radical scavenging; linear regression analysis; spectrophotometric approach
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Marxen, K.; Vanselow, K.H.; Lippemeier, S.; Hintze, R.; Ruser, A.; Hansen, U.-P. Determination of DPPH Radical Oxidation Caused by Methanolic Extracts of Some Microalgal Species by Linear Regression Analysis of Spectrophotometric Measurements. Sensors 2007, 7, 2080-2095.

AMA Style

Marxen K, Vanselow KH, Lippemeier S, Hintze R, Ruser A, Hansen U-P. Determination of DPPH Radical Oxidation Caused by Methanolic Extracts of Some Microalgal Species by Linear Regression Analysis of Spectrophotometric Measurements. Sensors. 2007; 7(10):2080-2095.

Chicago/Turabian Style

Marxen, Kai; Vanselow, Klaus H.; Lippemeier, Sebastian; Hintze, Ralf; Ruser, Andreas; Hansen, Ulf-Peter. 2007. "Determination of DPPH Radical Oxidation Caused by Methanolic Extracts of Some Microalgal Species by Linear Regression Analysis of Spectrophotometric Measurements." Sensors 7, no. 10: 2080-2095.



Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert