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Biosensing with Luminescent Semiconductor Quantum Dots
Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory, Washington, DC 20375-5320, USA
Division of Optical Sciences, Code 5611, U.S. Naval Research Laboratory, Washington, DC 20375-5320, USA.
George Mason University, 10910 University Blvd, MS 4E3, Manassas, VA 20110 USA.
Chemical & Biomolecular Eng. Dept. Johns Hopkins University, Baltimore, MD 21218 USA
* Authors to whom correspondence should be addressed.
Received: 12 April 2006 / Accepted: 9 June 2005 / Published: 24 August 2006
Abstract: Luminescent semiconductor nanocrystals or quantum dots (QDs) are a recentlydeveloped class of nanomaterial whose unique photophysical properties are helping tocreate a new generation of robust fluorescent biosensors. QD properties of interest forbiosensing include high quantum yields, broad absorption spectra coupled to narrow sizetunablephotoluminescent emissions and exceptional resistance to both photobleaching andchemical degradation. In this review, we examine the progress in adapting QDs for severalpredominantly in vitro biosensing applications including use in immunoassays, asgeneralized probes, in nucleic acid detection and fluorescence resonance energy transfer(FRET) - based sensing. We also describe several important considerations when workingwith QDs mainly centered on the choice of material(s) and appropriate strategies forattaching biomolecules to the QDs.
Keywords: Biosensor; fluorescence; antibody; DNA; detection; FRET; quantum dot; probe; immunoassay; nanocrystal.
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Cite This Article
MDPI and ACS Style
Sapsford, K.E.; Pons, T.; Medintz, I.L.; Mattoussi, H. Biosensing with Luminescent Semiconductor Quantum Dots. Sensors 2006, 6, 925-953.
Sapsford KE, Pons T, Medintz IL, Mattoussi H. Biosensing with Luminescent Semiconductor Quantum Dots. Sensors. 2006; 6(8):925-953.
Sapsford, Kim E.; Pons, Thomas; Medintz, Igor L.; Mattoussi, Hedi. 2006. "Biosensing with Luminescent Semiconductor Quantum Dots." Sensors 6, no. 8: 925-953.