Sensors 2006, 6(10), 1234-1244; doi:10.3390/s6101234
Article

Studies on the Interaction Mechanism of 1,10-Phenanthroline Cobalt(II) Complex with DNA and Preparation of Electrochemical DNA Biosensor

Shuyan Niu, Feng Li, Shusheng Zhang* email, Long Wang, Xuemei Li and Shiying Wang
College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.R. China
* Author to whom correspondence should be addressed.
Received: 1 September 2006 / Accepted: 5 October 2006 / Published: 10 October 2006
(This article belongs to the Special Issue Amperometric Sensors and Techniques for Neurochemical Monitoring)
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Abstract: Fluorescence spectroscopy and ultraviolet (UV) spectroscopy techniques coupled with cyclic voltammetry (CV) were used to study the interaction between salmon sperm DNA and 1,10-Phenanthroline cobalt(II) complex, [Co(phen)2(Cl)(H2O)]Cl·H2O, where phen = 1,10-phenanthroline. The interaction between [Co(phen)2(Cl)(H2O)]+ and double-strand DNA (dsDNA) was identified to be intercalative mode. An electrochemical DNA biosensor was developed by covalent immobilization of probe single-strand DNA (ssDNA) related to human immunodeficiency virus (HIV) on the activated glassy carbon electrode (GCE). With [Co(phen)2(Cl)(H2O)]+ being the novel electrochemical hybridization indicator, the selectivity of ssDNA-modified electrode was investigated and selective detection of complementary ssDNA was achieved using differential pulse voltammetry (DPV).
Keywords: 1; 10-Phenanthroline; Cobalt(II); Voltammetry; Intercalative; Electrochemical DNA biosensor

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MDPI and ACS Style

Niu, S.; Li, F.; Zhang, S.; Wang, L.; Li, X.; Wang, S. Studies on the Interaction Mechanism of 1,10-Phenanthroline Cobalt(II) Complex with DNA and Preparation of Electrochemical DNA Biosensor. Sensors 2006, 6, 1234-1244.

AMA Style

Niu S, Li F, Zhang S, Wang L, Li X, Wang S. Studies on the Interaction Mechanism of 1,10-Phenanthroline Cobalt(II) Complex with DNA and Preparation of Electrochemical DNA Biosensor. Sensors. 2006; 6(10):1234-1244.

Chicago/Turabian Style

Niu, Shuyan; Li, Feng; Zhang, Shusheng; Wang, Long; Li, Xuemei; Wang, Shiying. 2006. "Studies on the Interaction Mechanism of 1,10-Phenanthroline Cobalt(II) Complex with DNA and Preparation of Electrochemical DNA Biosensor." Sensors 6, no. 10: 1234-1244.

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