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Sensors 2006, 6(10), 1234-1244; doi:10.3390/s6101234
Article

Studies on the Interaction Mechanism of 1,10-Phenanthroline Cobalt(II) Complex with DNA and Preparation of Electrochemical DNA Biosensor

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Received: 1 September 2006; Accepted: 5 October 2006 / Published: 10 October 2006
(This article belongs to the Special Issue Amperometric Sensors and Techniques for Neurochemical Monitoring)
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Abstract: Fluorescence spectroscopy and ultraviolet (UV) spectroscopy techniques coupled with cyclic voltammetry (CV) were used to study the interaction between salmon sperm DNA and 1,10-Phenanthroline cobalt(II) complex, [Co(phen)2(Cl)(H2O)]Cl·H2O, where phen = 1,10-phenanthroline. The interaction between [Co(phen)2(Cl)(H2O)]+ and double-strand DNA (dsDNA) was identified to be intercalative mode. An electrochemical DNA biosensor was developed by covalent immobilization of probe single-strand DNA (ssDNA) related to human immunodeficiency virus (HIV) on the activated glassy carbon electrode (GCE). With [Co(phen)2(Cl)(H2O)]+ being the novel electrochemical hybridization indicator, the selectivity of ssDNA-modified electrode was investigated and selective detection of complementary ssDNA was achieved using differential pulse voltammetry (DPV).
Keywords: 1; 10-Phenanthroline; Cobalt(II); Voltammetry; Intercalative; Electrochemical DNA biosensor 1; 10-Phenanthroline; Cobalt(II); Voltammetry; Intercalative; Electrochemical DNA biosensor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Niu, S.; Li, F.; Zhang, S.; Wang, L.; Li, X.; Wang, S. Studies on the Interaction Mechanism of 1,10-Phenanthroline Cobalt(II) Complex with DNA and Preparation of Electrochemical DNA Biosensor. Sensors 2006, 6, 1234-1244.

AMA Style

Niu S, Li F, Zhang S, Wang L, Li X, Wang S. Studies on the Interaction Mechanism of 1,10-Phenanthroline Cobalt(II) Complex with DNA and Preparation of Electrochemical DNA Biosensor. Sensors. 2006; 6(10):1234-1244.

Chicago/Turabian Style

Niu, Shuyan; Li, Feng; Zhang, Shusheng; Wang, Long; Li, Xuemei; Wang, Shiying. 2006. "Studies on the Interaction Mechanism of 1,10-Phenanthroline Cobalt(II) Complex with DNA and Preparation of Electrochemical DNA Biosensor." Sensors 6, no. 10: 1234-1244.



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